摘要
以“珲春桃”、“正满早生”及其32株F1杂种为试材,对RAPD标记的分离方式进行了研究。结果表明:10个10碱基引物共扩增出57个RAPD标记,这些标记在F1代的分离有孟德尔分离、偏离孟德尔分离和异常分离3种方式。3种分离方式出现的频率分别为87.7%,10.5%和1.8%。在符合孟德尔分离的标记中,不分离标记有30条,占60.0%,是孟德尔分离标记的主体;符合1∶1或3∶1分离比例的有20条,占40.0%,平均每个引物产生2.0个此类标记,可用于构建遗传连锁图谱。本研究结果表明该群体适合利用RAPD标记构建遗传连锁图谱。
Hunchuntao, zhengmanzaosheng and their F1 hybrids were used as materials to study the segregation patterns of RAPD markers. The results indicated that total 57 RAPD markers were generated by ten 10-mer primers, and these markers＇ segregateion patterns could be divided into three types： Mendelian segregation, deviation from Mendelian segregation and abnormal segregation. Frequencies of the three types in all bands were 87.7%, 10.5% and 1.8%, respectively. Among these markers of Mendelian segregation, there existed 60% of non-segregation marker, 40% of 1 ： 1 or 3：1 segregation marker. Average 2.0 markers of 1 ： 1 or 3：1 segregation were generated by each primer, these markers could be used for constructing genetic linkage map. It also indicated that the F1 population was suitable for constructing genetic linkage map.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第4期402-406,共5页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30160054)
关键词
抗寒桃
杂种一代
RAPD标记
分离方式
cold-resistant peach
F1 hybrid
RAPD marker
segregation pattern
