摘要
目的建立可用于中国的蚊虫携带西尼罗病毒(WNV)监测的快速检测方法。方法选择两种快速检测方法VecTest试剂盒和Ramp系统,对其检测WNV的敏感性、精确性以及与流行性乙型脑炎(乙脑)病毒的交叉反应进行研究,同时对采自北京市野外的蚊虫进行WNV检测。结果Ramp试剂盒检测方法比VecTest试剂盒方法敏感性高,但后者检测时间短、操作简便,更适合现场筛查使用;经RT-PCR方法确认此2种快速检测方法无假阳性及假阴性结果;VecTest试剂盒和Ramp系统与乙脑病毒也无交叉反应。使用这2种检测方法对北京市6种野外蚊虫进行了WNV检测,结果均为阴性。结论VecTest试剂盒和Ramp系统均可用于WNV快速检测。
Objective To select West Nile virus（WNV） quick detecting methods from mosquitoes which can be used in China. Methods Two methods, ADAPCO＇s RAMP System and MAS＇s VecTest kits were selected. The sensitivity and accuracy of these two methods and its cross-reaction with Japanese Encephalitis virus were tested. These two methods also were used to detect WNV in wild mosquitoes. Results The results show that both of the two methods can be used to detect WNV. Ramp system is more sensitive than VecTest kits. RT-PCR confirmed that no false positive and false negative results were obtained. These two methods have no cross-reaction in detecting WNV and Japanese Encephalitis virus. 6 species of wild mosquitoes were detected by these two methods. No WNV was found. Conclusion Both of Ramp system and VecTest can be used to detect WNV.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2006年第4期307-310,共4页
Chinese Journal of Vector Biology and Control
