摘要
目的建立消栓滴丸中芍药苷的含量测定方法。方法采用高效液相色谱(HPLC)法,对消栓滴丸中芍药苷提取溶剂、时间进行了优选,以LichrospherC18柱(5μm,150mm×4.6mm)为分析柱;流动相为甲醇-水(30∶70);检测波长:230nm;流速:1.0mL/min;柱温:室温。结果样品提取工艺为消栓滴丸粉用甲醇超声处理40min。芍药苷线性范围为0.051～0.510mg/mL,平均回收率98.37%,RSD=3.13%。结论该方法操作简便,专属性强,可用于消栓滴丸的质量控制。
Objective To establish the determination method of Paeoniflorin in Xiaoshuan pills. Methods Extract solvent and time was studied by HPLC method. The HPLC system consisted of Lichrospher C18 column （5μm,150 mm×4.6 mm） and methanol-water （30 ： 70） as the mobile phase. The detective wavelength was 230 nm, the flow rate was 1.0 mL/min, and the temperature was 25℃. Results The powder of Xiaoshuan pills was extracted with methanol. The Paeoniflorin was linear within the range of 0.051-0.510 mg/mL, the average recovery was 98.37%, and RSD was 3.13%. Conclusion The method is simple, highly exclusive, and suitable for quality control of Xiaoshuan pills.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第8期38-39,共2页
Chinese Journal of Information on Traditional Chinese Medicine
