摘要
目的扩增人蛲虫(Enterobius vermicularis)核糖体DNA的第一内转录间隔区(ITS1)。方法提取人蛲虫的基因组DNA,PCR扩增rDNA的ITS1及部分5.8S片段,对该片段进行PCR-SSCP分析并测序。结果扩增的片段大小为334bp,包含Enterobius vermicularis全部的ITS-1(303bp)及部分5.8S(31bp)序列,ITS1种内序列一致。结论首次报道人蛲虫的ITS1序列,将为以ITS1作为遗传标记用于蛲虫鉴别诊断、系统发育等研究奠定基础。
Objective To obtain the sequences of ITS1 of Enternbius vermicularis. Methods The internal transcribed spacer 1 （ITS1） and partial 5.8S rDNA of E. vermicularis were amplified by PCR and analysed by single - strand conformation polymorphism （SSCP）. Results No variation in single - stranded ITS1 profile was detected among samples, and complete ITS1 was 303bp, and partial 5.8S was 31 bp. Conclusion It was the first time that the complete sequence of ITS1 rDNA of E. vermicularis was reported .The results of the present study indicate ITS data may be useful for the diagnosis of E. vermicularis infection and the investigation of phylogenetics.
出处
《中国热带医学》
CAS
2006年第8期1336-1337,共2页
China Tropical Medicine
