摘要
通过软琼脂克隆法及有限稀释法,从(Hca-F25/L)细胞系中分离出五株不同转移力的克隆细胞,它们的共同特点是:均沿淋巴系统转移,仅有极少数(1.88%)同时伴有肺转移;五株克隆及其亲代对角蛋白单抗(HK2)均呈现阳性,提示尽管它们分化很低,但仍保持了上皮组织的特性;经染色体分析证实,每一克隆内同一核型的细胞占绝大多数,它们和亲代有相同的四个标记染色体,表明它们之间有“血缘”关系。它们的不同之处表现在:在615小鼠体内均能致瘤(100%),但在淋巴结中的转移率及其转移程度有很大的差别,其中一株高转移细胞系(78.6%—89.4%),三株中转移细胞系(33.3%—66.6%),一株低转移细胞系(15%～15.4%);五株克隆及其亲代的干系核型也各不相同;在细胞形态上和细胞电泳率方面,高、低转移细胞系与亲代细胞系三者之间有明显的差别。本文较确切地证明了 Hca 瘤株在转移表型和核型上的异质性。同时,也补充和发展了 H22/Hca 肿瘤系统。
By means of soft agar clonization and limited diluting methods,5 cell clone with different metastatic capacity have been separated from HcaF25/L cell line.Their common charaters are:All clone metastasize
along lymphatic system except a small percent (1.88%) associated with lung metastasis;5 cell clone as well as the parental generation all present keratin monoclonal antibody (HK2) positive reaction,indicate epithelial character with low differentiation;karyotype assay reveal each clone has highly identical karyotype,though they are different
from each other but they have simillar marker chromosomes (M_1,M_2,M_3,M_4).The different among them are:various lymphatic metastatic capacity,say,one highly metastatic clone (78.6—89.4%),three moderately metastatic clone (33.3—66.6%)and one low metastatic clone(15—15.4%);the three types cell clone has various morphology and different cell electrophoretic rate.All the results indicated that Hca strain is heterogeneous in phenotype and karyotype.
基金
国家自然科学基金资助项目
关键词
肿瘤转移
克隆细胞系
Hca-F25/L
Hca-F25/L
cloning
subpopulation
lymphatic metastasis
karyotype analysis
