摘要
我们采用蛋白酶（０．２ｍｇ／ｍｌ）循环消化的方法获取了７０％～８０％耐钙豚鼠心肌细胞，并用膜片钳全细胞式记录方法，初步观察了卡托普利（Ｃａｐｔ）对心室肌细胞跨膜电位及Ｌ型钙电流的影响。实验表明，Ｃａｐｔ１０μｍｏｌ／Ｌ显著缩短豚鼠心肌单细胞的动作电位时程（ＡＰＤ），药物作用１０分钟后，ＡＰＤ５０缩短３７．２２％，ＡＰＤ９０缩短３５．３３％（ｎ＝３，Ｐ＜０．０５）；但对静息电位（ＲＰ）、超射（ＯＳ）和动作电位幅度（ＡＰＡ）无显著影响。当心肌细胞的保持电压为－４０ｍＶ，除极到０ｍＶ，刺激电压时程为２５０ｍｓ，频率为０．５Ｈｚ时，Ｃａｐｔ１０μｍｏｌ／Ｌ可使Ｌ型钙内向峰电流减少４２．６８％，正常对照组在同一时间内仅下降４．１％（ｎ＝３，Ｐ＜０．０５）。可见，卡托普利具有类似维拉帕米样Ｌ－型钙通道阻断剂作用。
The effect of captopril (Capt) on transmembrane potential and L type Ca 2+ channel current in guinea pig single ventricular myocytes was studied using patch clamp whole cell recording technique.The results showed that capt markedly shortened the action potential duration (APD);had no significant effct on rest potential(RP),over shoot (OS) and action potential amplitude (APA). When the holding potnetial was -40 mV, cells were depolarized to 0mV for 250ms at frequency of 0.5Hz, Capt 10μmol/L reduced verapamil sensitive Ca 2+ inward current by 42.68%. This indicated that the capt has blocking effect on the L type calcium channels.
出处
《哈尔滨医科大学学报》
CAS
1996年第4期322-325,共4页
Journal of Harbin Medical University
关键词
卡托普利
动作电位
L-型钙电流
心室肌细胞
Captopril
Action pontential
L type Ca current
Patch clamp
Guinea pig
Ventricular myocytes
