摘要
考察了在双底物抑制下脂肪酶催化合成乳酸乙酯,最佳溶剂为叔丁醇,在所选的几种脂肪酶中,固定化于大孔丙烯酸树脂的南极假丝酵母脂肪酶B(Novozym435)的催化活性最好.对反应条件进行了优化,当酸醇摩尔比为1∶8,反应温度60℃,酸浓度0·3mol/L,酶浓度45g/mol,摇床转速200r/min时产率达到77%,脂肪酶Novozym435重复使用6次后产率仍然可达到60%.
Esterification of lactic acid and ethanol by lipase under double substrate inhibition was studied. The best solvent is tert-butyl alcohol and the best lipase is Novozym 435 （immobilized on a macroporous acrylic resin） for this reaction system. The optimization of reaction conditions was studied in detail. When the molar ratio of acid to alcohol is 1 ： 8, the temperature is 60 ℃, the concentration of acid and lipase is 0.3 mol/L and 45 g/mol, and the revolution is 200 r/min, a yield of 77 % was reached. Novozym 435 has a high stability. A yield of 60 % for ethyl lactate can be obtained with Novozvm 435 even after it was used continuously six times.
出处
《催化学报》
SCIE
CAS
CSCD
北大核心
2006年第6期537-540,共4页
基金
河北省自然科学基金资助项目(200014)
关键词
脂肪酶
乳酸
乙醇
酯化
乳酸乙酯
双底物抑制
lipase
lactic acid
ethanol
esterification
ethyl lactate
double substrates inhibition
