摘要
目的探讨14种中草药对白念珠菌的抗菌作用。方法将黄连、黄柏、虎杖、丁香、石榴皮、七叶一枝花、五倍子、白头翁、仙鹤草、连翘、金银花、苦参、蛇床子、诃子等14味中药材制备成醇浸膏,采用倍比稀释法,将醇浸膏分别稀释成1 000.00,500.00,250.00,125.00,62.50,31.25 mg.mL-1的溶液,采用平板打孔法和平板稀释法分别测定14种中草药体外抗白念珠菌的效果。结果黄连的最小抑菌浓度为6.25 mg.mL-1,黄柏、虎杖的最小抑菌浓度为25.00mg.mL-1,丁香、石榴皮、七叶一枝花、五倍子的最小抑菌浓度为50.00 mg.mL-1。未见其他药物体外对白念珠菌有明显抑制作用。结论黄连醇浸膏体外对白念珠菌有明显抑制作用,虎杖、黄柏、丁香、石榴皮、七叶一枝花、五倍子的醇浸膏体外对白念珠菌有一定的抑菌作用。
Objective To study the in vitro antimicrobial effects of 14 Chinese herbal medicines （CHMs） on Candida albicans. Methods Alcoholic extracts of the following 14 CHMs were prepared： coptis root, Phelledendron bark, giant knotweed rhizome, clove, pomegranate rind, Chinensis Franch, Chinese gall, pulsatilla root, hairy vein agrimony, forsythia root, honey suckle flower, flavescent sophora root, cnidium fruit and cbebula fruit. The extracts were then diluted into solutions containing 1 000. 00, 500. 00, 250. 00, 125.00, 62.50, 31.25 mg·mL^-1 of the cuude drug, respectively. The in vitro antimicrobial effects of the 14 CHMs on Candida allicans were determined with the disk diffusion method and disk dilution method. Results The minimal inhibitory concentration （MIC） of coptis root was 6.25 mg·mL^-1. The MIC of Phelledendron bark and giant knotweed rhizome was 25.00 mg ·mL^-1. The MIC of clove, pomegranate rind, Chinensis Franch and Chinese gall was 50：00 mg·mL^-1. The remaining CHMs were shown to have no apparent inhibitory effect on Candida albicans in vitro. Conclusion Among the 14 CHMs, coptis roots was shown to have the strongest in vitro inhibitory effect on Candida albicans. Giant knotweed rhizome, Phellondendron bark, clove, pomegranate rind, Chinensis Franch and Chinese gall had also in vitro inhibitory effects on the microbe to a certain degree.
出处
《医药导报》
CAS
2006年第8期765-767,共3页
Herald of Medicine
关键词
中草药
白念珠菌
最小抑菌浓度
Chinese herbal medicine
Candida albicans
Minimal inhibitory concentration （MIC）
