摘要
目的探讨乙醇对大鼠睾丸细胞凋亡的影响。方法将72只雄性性成熟大鼠随机分为3组,对照组24只给予0.9%氯化钠溶液灌胃,6 mL.kg-1.d-1;实验组A和实验组B分别均给予50度乙醇(乙醇浓度为0.378g.mL-1)灌胃,剂量分别为6和12 mL.kg-1.d-1。均连续灌胃39 d。分别在实验第14,27,40天取各组大鼠睾丸细胞,采用流式细胞仪结合FITC-Annexin V/PI荧光染色检测大鼠睾丸细胞凋亡情况。结果第14,27,40天,对照组大鼠睾丸细胞凋亡指数(AI)分别为(7.41±1.97)%,(9.06±2.36)%和(8.58±1.64)%,不同时点差异无显著性(均P>0.05)。实验组A第27,40天平均AI分别为(48.26±6.27)%和(53.18±6.56)%,差异无显著性(P>0.05),但均比第14天[(13.07±2.05)%]显著增高(均P<0.01);实验组B第27,40天AI分别为(53.41±6.93)%和(59.89±7.05)%,差异无显著性(P>0.05),但均比第14天[(19.68±3.84)%]高(均P<0.01)。实验组A和实验组B大鼠AI在各时间点均较对照组大鼠高(均P<0.01)。实验组B第14天AI明显较实验组A高(P<0.01)。结论乙醇可导致大鼠睾丸细胞凋亡增加。灌胃给予乙醇,随着时间的延长,大鼠睾丸细胞凋亡增加;27 d后睾丸细胞的凋亡保持较高水平。
Objective To study the effect of alcohol on the testicular cell apoptosis in the rat. Methods 72 adult male Wistar rats were randomly divided into 3 equal groups. Rats of the control group were given each 6 mL· kg^-1 of 0.9% sodium chloride solution administered by gastrogavage q. d.. Rats of the trial group A and trial group B were given each 6 mL· kg^-1 and 12 mL· kg^-1 of alcohol（50% alcohol, containing 0. 378 g of ethanol per mL）, respectively, administered by gastrogavage q. d.. The course of treatment in all 3 groups lasted 39 days. On each of the 14^th, 27^th and 40^th day of the experiment, 8 rats from each of the 3 groups were sacrificed by cervical dislocation and the testes were removed for the assay of testicular cell apoptosis with flow cytometry（FCM） and fluorescent staining （FITC-Arnexin V/PI）. Results Testicular cell apoptosis indexes （AI） in rats of the control group on the days 14,27and 40 were （7.41 ± 1.97）%, （9.06 ±2.36）% and （8.58 ± 1.64）% respectively, differences between these values being insignificant （P 〉 0.05 ）. The Als in rats of the trial group A on the days 27 and 40 were （48.26 ±6.27）% and （53.18 ±6.56）% ,respectively,the difference being insignificant as well （ P 〉 0.05 ）. However, they were significantly higher than the AI on the dayl 4 （ 13.07 ± 2.05 ） % （ P 〈 0.01 ）. The AIs in rats of the trial group B on the days 27 and 40 were （ 53.41 ± 6.93 ） % and （ 59.89 ± 7.05 ） %, respectively, the difference being insignificant（ P 〉 0.05）. They were, however, significantly higher than the AI on the day 14 （19.68 ± 3.84 ）% （P 〈 0.01 ）. AI in rats of beth the trial groups A and B on the days 14,27 and 40 were significantly higher than those in rats of the control group on the corresponding days, respectively （ P 〈 0.01 ）. The AI in rats of the trial group B was significantly higher than that in rats of the trial group A on the day 14（ P 〈 0.01 ）. Conclusion Alcohol administered by gastrogavage was shown to increase the apoptosis of testicular cells in the rat in a dosage and time-dependant manner in the first 27 days, and the extent of the apoptosis then became stable within 40 days.
出处
《医药导报》
CAS
2006年第8期748-751,共4页
Herald of Medicine
