摘要
根据牛SRY基因序列设计合成2对巢式PCR引物作为性别鉴定引物,根据牛-珠蛋白基因序列设计了一对引物作为内标引物建立了牛胚胎性别鉴定的PCR反应体系。公牛可以扩增出187bp的SRY基因片段和255 bp的-珠蛋白基因片段,母牛只能扩增出255 bp的-珠蛋白基因片段。由于巢式PCR只需3～8个细胞就可以在紫外灯下看到扩增结果,而常规PCR则需要较多的细胞,所以胚胎性别鉴定时使用巢式PCR效果更好。
We designed 2 pairs of SRY gene nested primers for sex determination and a pair off β-globin gene primer as an internal standard in this study and established the system ofPCR. As a result, 187 bp and 255 bp bands could be visible on the gel in ultraviolet transilluminator for male, but for female ,only 255 bp band could be visible. It needed only 3 to 8 cells for obtaining amplification result by nested PCR, but more cells were needed by normal PCR.So we could obtain better results of sex determination of bovine preimplantation embryos by nested PCR.
出处
《吉林畜牧兽医》
2006年第8期8-10,共3页
Jilin Animal Husbandry and Veterinary Medicine
关键词
牛
早期胚胎
性别鉴定
巢式PCR
Bovine. Early embryo. Sex determination. Nested PCR
