摘要
用安捷伦Zorbax SB-C18（4．6cm×250cm，5μm）色谱柱，以K2HPO4（0．02mol／L，pH值3）和甲醇（二者体积比为90：10）为流动相，在紫外检测波长218nm的条件下，利用反相高效液相色谱法（RP-HPLC）测定发酵液中庚二酸含量，获得的庚二酸吸收峰面积与质量浓度的线性相关系数为0．999。庚二酸加标回收率98．38％．101．84％，相对标准偏差为0．45％-1．0％。该方法能准确测定发酵液中庚二酸含量，而且操作简便、快捷、结果可靠。
A method for determining pimelic acid in the broth system by reversed-phase high performance liquid chromatography（RP-HPLC） was developed.The HPLC condition were as follows：Aglilent Zorbax SB-C18（4.6 cm×250cm,5μm） with a mixture of methanol（10%）and 0.02 mol/L, pH 3.0 K2HPO4 [ 90 % （ V/V ） ] as the mobile phase; UV detection wavelength is at 218 nm. The linear range of pimelic acid was 0. 999, The recoveries were 98.38% - 101.84% with relative standard deviation 0.45% - 1.0% .The method is simple ,rapid and accurate for determining pimelic acid in broth system.
出处
《安徽农业科学》
CAS
北大核心
2006年第13期2952-2953,2955,共3页
Journal of Anhui Agricultural Sciences
