摘要
利用构建的含潮霉素(HygB)抗性标记的质粒pUCATPH在稻瘟病菌菌株M 131中建立一个转化体系,通过限制酶介导整合(REM I)插入诱变技术,以HygB抗性作为突变体筛选标记,获得639个转化子。对其中200个转化子进行表型和致病性测定后,通过点杂交鉴定分别获得6个致病性突变菌株和部分表型突变菌株。对2个表型突变菌株和rep-PCR图谱差异性较大的2个致病性突变菌株进行RFLP分析,结果表明:突变体中均已插入质粒,但转化质粒在M 131中整合具有一定的随机性。
The plasmid pUCATPH was used to establish a transformation system in isolate M131 of Magnaporthe grisea. 639 transformants were obtained by restriction enzyme mediated integration with Hygromycin 13 resistance as a tag. Two pbenotype mutants and six pathogenicity mutants were screened out respectively, through the detection of the pbenotype and pathogenicity and dot blot of 200 transformants, rep-PCR and RFLP analyses were done to study the mutation on molecular level and the integration sites of the plasmid DNA in the four mutants. Result showed that plasmid was inserted in all mutants and the conformity was random.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2006年第2期91-94,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(39870429)
国家"十五"科技攻关项目(2004BA520A1501)
