摘要
①目的研究从人胎盘中提纯一种低分子量的肝细胞生长因子的实验方法及该因子的某些特性。②方法人胎盘匀浆液经高速离心后，上清液经分段盐析、阴离子交换层析、超滤和肝素亲和层析，纯化约６００～８００倍。③结果该生长因子是一种对热稳定、亲和肝素的蛋白质，分子量为１６．８７ｋｕ，能促进原代培养的成年大鼠肝细胞的ＤＮＡ合成，其作用有剂量依赖关系，并能抑制小鼠Ｓ１８０细胞的ＤＮＡ合成。④结论人胎盘中存在一种特殊的肝细胞生长因子。
Objective A kind of hepatocyte growth factor with low molecular weight from human placenta was purified and its characteristics were studied. Method The homogenized solution of human placenta was centrifuged at high speed and the supernatant was subjected to ammonium sulfate precipitation. DEAE sepharose anion exchange chromatography, ultrafiltration and heparin affinity chromatography. Finally, the factor was purified 600￣800 fold. Result The factor, which was different from hepatocyte growth factor from body fluid(HGF h) and hepatocyte growth factor from liver cell sol(HGF c), was a heat stable protein with affinity to heparin. Its molecular weight was 16.87KU. Not only could it stimulate DNA synthesis of adult rat hepatocytes in primary cell cultrue, but also inhibit DNA synthesis of S 180 cells in vitro. The effect of the factor on hepatocytes was dose dependent. Conclusion There was a kind of special hepatocyte growth factor in human placenta.
出处
《青岛医学院学报》
CAS
1996年第2期118-120,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
肝细胞生长因子
胎盘多肽
人
提纯
细胞学技术
hepatocyte growth factor
placental peptide
men
purification
cytological technics
