摘要
目的探讨大鼠C6脑胶质瘤模型冷冻治疗后细胞凋亡水平和增殖活性的变化。方法借助于立体定位技术,建立大鼠C6皮层脑胶质瘤动物模型,待肿瘤生长至第15天、直径约6mm时,进行冷冻治疗。治疗后15d行细胞凋亡的末端标记法(TUNEL)和流式细胞仪(FCM)检测,以增殖细胞核抗原(PCNA)免疫组织化学及MRI检查了解肿瘤的增殖活性,测量肿瘤体积和抑瘤率。结果冷冻治疗后凋亡细胞密度及凋亡指数较对照组显著提高[前者分别为(36.73±9.54)/0.0625mm2和(4.87±2.80)/0.0625mm2;后者分别为(16.02±3.87)%和(1.70±1.74)%](P<0.01)。冷冻组肿瘤内PCNA阳性细胞数量较对照组显著下降[分别为(42.37±7.63)%和(73.93±9.60)%](P<0.01);肿瘤体积明显缩小[分别为(139.60±27.28)mm3和(414.40±69.01)mm3](P<0.01),增殖受抑制,抑瘤率为66.31%。结论大鼠C6脑胶质瘤冷冻治疗后除细胞坏死外,肿瘤细胞增殖水平下降和细胞凋亡增多也是冷冻治疗胶质瘤的机理之一。
Objective To explore the changes in apoptosis level and proliferative activity of the C6 brain glioma cells after cryotherapy in rats. Methods The C6 cortex brain glioma model was established in rats by stereotaxic technique. When the tumor diameter was some 6 mm 15 days after the inoculation, 60 rats were randomly divided into control group and cryotherapy group, in which cryotheraphy was performed. The apoptosis was detected by TUNEL and flow cytometry, proliferating cell neuclear antigen （PCNA） was determined by immunohistochemistry, and the proliferative activity was evaluated by PCNA and MRI 15 days after the cryotherapy. The tumor volume was measured and tumor growth inhibition rate was computed. Results The density and index of apoptosis cells in the eryotherapy group were （36.73±9.54）/0.0625mm^2 and （16.02%±3.87%） respoetively and they in the control group were （4.87±2.80）/0.0625mm^2 and （1.70±1.74）% respectively. There was significant difference in the density and index of apoptosis cells between both the groups （P〈0.01）. The percentage of positive cell number of PCNA in the cryotherapy group （42.37%±7.63%） was significantly fewer than that （73.93%±9.60%） in the control group （P〈0.01）. And the tumor volume （139.60mm^3±27.28mm^3） in the cryotherapy group was significantly smaller than that （414.40mm^3±69.01mm^3） in the control group （P〈0.01）. The inhibition rate of the tumor in the cryotherapy group was 66.31%. Conclusion Besides the necrosis, the decrease in the proliferative level and the increase in the apoptosis cells may also be one of the mechanisms by which cryotherapy inhibits the tumor growing.
出处
《中国临床神经外科杂志》
2006年第7期410-413,共4页
Chinese Journal of Clinical Neurosurgery
基金
湖北省教育厅重点科研基金资助项目(No.2003A007)
湖北省科技厅科技攻关项目(No.2003AA301C56)
十堰市科技局科技攻关项目(No.2003041D)
