摘要
采用免疫组化和原位分子杂交技术观察ｐ５３、Ｈ－ｒａｓ、ｃ－ｅｒｂＢ－２、ｃ－ｍｙｃ癌基因和抗癌基因及其蛋白产物在原发性食管腺癌（ＰＥＡ）中的联合表达情况，以探讨ＰＥＡ的发生发展机制。结果表明，ＰＥＡ中的ｐ５３表达较食管鳞癌高；ｃ－ｅｒｂＢ－２变化可能是ＰＥＡ癌变的重要分子学改变；ＰＥＡ中Ｈ－ｒａｓ与ｃ－ｍｙｃ都有基因扩增蛋白产物的过表达，显示两者在ＰＥＡ的癌变过程中有协同作用；ｐ５３、ｃ－ｅｒｂＢ－２的表达与ＰＥＡ的分化程度呈负相关，即分化程度越差，阳性率越高；ＰＥＡ中癌基因和抗癌基因在基因水平及其蛋白产物表达上的改变基本一致。
The comparision of the expression of the oncogene H-ras、c-erbB-2、c-myc and p53 antioncogene,and the expression of their protein products in all subtypes of PEA were studied by in-situ hybridization and immunohistochemistry. The results showed that the expression of p53 was higher in PEA than that in squamous cell carcinoma of the esophagus.The c-erbB-2 might be the important molecular change of PEA.H-ras and c-myc were expressed and amplified excessively in PEA, It suggested that H-ras and c-myc might play a cooperative role,The expression of p53 and c-erbB-2 seemed have an inverse ratio to the differentiation of PEA,The lower the tumor differentiation, the higher the positive rate. The expression of oncogene、 antioncogene and their protein products in PEA were consistent.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
1996年第3期200-202,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
高等学校博士点专项基金
美国NCI基金
关键词
食管肿瘤
腺癌
癌基因
抗癌基因
免疫组织化学
primary esophageal adenocarcinoma
in-situ hybridization
immunohistochemistry
oncogene
antioncogene
