摘要
目的:了解乳腺干细胞在不同性质的乳腺组织中的含量。方法:取得手术后不同性质的乳腺组织,用胶原酶消化法制备成单细胞悬液后,采用流式细胞技术测定乳腺细胞的CD44和CD24表达情况。结果:从正常、乳腺疾病到乳腺癌组织,CD44+CD24-/low乳腺细胞的比例上升;当乳腺癌细胞生长加速时,CD44+CD24+乳腺细胞比例明显增加。结论:乳腺干细胞的含量可以反映乳腺组织的性质,而CD44+CD24+乳腺细胞可能是祖细胞,并且其含量可以反映乳腺组织的生长情况。
Objective： To know the proportion of mammary stem cell in different mammary tissue. Methods： obtaioned differcur mammary tissue after operation and then prepared single cell suspensions by collagenase, and measured the expression of CD44 and CD24 of the mammary cells. Results： From normal, benign to malignant mammary tissues, the proporation of CD44^＋ CD24^-/low of the mammary cells gradually increased. When proliferation of mammaty cancer cells was accelerated, the proporation of CD44^＋ CD24^＋ of the mammary cell also markedly increased. Conclusions： The proporation of mammary stem cells could reflect the property of mammary tissue. CD44^＋ CD24^＋ of the mammary cells were mammary progenitor cell, and its proporafion could reflect the proliferation of mammary tissue.
出处
《华西医学》
CAS
2006年第3期442-444,共3页
West China Medical Journal
关键词
干细胞
祖细胞
乳腺癌
自我更新
stem cell
progenitor cell
breast cancer
self- renewal
