摘要
比较不同阿米巴的毒力。选择急性阿米巴痢疾患者体内分离的溶组织内阿米巴原虫（Ｅｈ株）、体外培养多年的Ｅｈ的Ｌａｒｅｄｏ株（Ｅｈ－Ｌａｒｅｄｏ株）和侵入内阿米巴原虫（Ｅｉ），分别与人、兔的红细胞３７℃孵育，在孵育后１ｈ、３ｈ和５ｈ观察它们吞噬红细胞的能力；以阿米巴滋养体与小鼠腹腔白细胞进行毒性试验。结果：Ｅｈ吞噬红细胞的能力显著高于其他两种阿米巴原虫（Ｐ＜０．０１）；Ｅｈ－Ｌａｒｅｄｏ株在２２℃和３７℃两种温度下对红细胞的吞噬能力并无差异；Ｅｉ株在一定的培养时间内对红细胞的吞噬活力随培养时间延长而增加。结论：阿米巴原虫均具有吞噬红细胞的能力，但其中以致病性的Ｅｈ对红细胞的吞噬力强，Ｅｈ株对白细胞的毒性显著高于Ｌａｒｅｄｏ株和Ｅｉ株。
PURPOSE In order to compare the virulence of different strains of amoeba, we isolated Entamoeba histolytica from patients with acute amoebic dysentery, E. histolytica-like Laredo strain and E. invadens.METHODS Three species above mentioned were incubated with human and rabbit erythyocytes at 37℃ respectively.After incubated,the erythrocytophagous quantification and ability of three species amoebas were evaluated by microscopy at 1 h￣3 h and 5 h.RESULTS There was no difference in the erythrocytophagous rate at 37℃ and 22℃ for Laredo strain as time goes on,E invadens shown increase of average number of human erythrocytes per amoeba. The leucocytoxicity effect test of the different kinds amoebas to mouse peritoneal leukocytes were used as an assay for determination of amebic virulence. The trophozoites from E.hiscolytica had markedly higher virulence to leukocytes than Lared strain and E invadens (P<0.01), and E.histolytica shown stronger.Erythrocytophogous effect than another amoeba (P<0.01). CONCLUSIONS The results suggested a signigicant erythrocytophagous activity of pathogenetic E.histolytica, however all amoebas are able to phagocytize erythrocytes.
出处
《上海医科大学学报》
CSCD
1996年第6期439-442,共4页
Journal of Fudan University(Medical Science)
关键词
阿米巴原虫
Laredo株
吞噬活性
白细胞毒性
Entamoeba histolytica Laredo strain Entamoeba invadens phagocytosis activity leucocytoxicity test
