摘要
目的:检测环上颌骨骨缝区增殖细胞核抗原阳性表达,探讨缝牵张促进上颌骨生长发育的作用。方法:实验于2005-03/09在解放军第四军医大学口腔医学院颌面外科实验室完成。15周龄杂种犬12只,随机数字法分为1周实验组4只,1周对照组2只,4周实验组4只,4周对照组2只。实验组安置口外自制牵引支架,自鼻腭孔引出牵引钩,橡皮圈连接牵引支架和牵引钩,分别持续弹性牵引1周和4周,牵引力约600g。牵引结束时将4组犬处死,取4组犬的前颌缝,腭横缝,颧颌缝,颧颞缝区组织,抗增殖细胞核抗原单克隆抗体免疫组织染色,检测骨缝区增殖细胞的分布及数量,进行统计学分析。结果:实验犬12只均存活至预定观察期。①1周实验组腭横缝、颧颌缝增殖细胞核抗原阳性表达高于1周对照组(1周实验组:腭横缝为48.69±5.42,颧颌缝为46.44±3.72;1周对照组:腭横缝为34.00±3.26,颧颌缝为33.75±5.58,P<0.05)。②4周实验组前颌缝、腭横缝、颧颌缝、颧颞缝增殖细胞核抗原阳性表达均高于4周对照组及1周实验组(4周实验组:前颌缝为70.78±3.58,腭横缝为69.9±2.62,颧颌缝为68.56±4.15,颧颞缝为64.25±2.82;4周对照组:前颌缝为32.95±3.24,腭横缝为34.83±5.68,颧颌缝为34.26±3.37,颧颞缝为33.46±3.47;1周实验组:前颌缝为38.97±4.25,腭横缝为48.69±5.42,颧颌缝为46.44±3.72,颧颞缝为36.06±4.34,P<0.05)。结论:缝牵张过程中,上颌骨周围骨缝增殖细胞数和活跃程度明显增加,提示缝牵张可促进上颌骨生长发育。
AIM：To detect proliferating cell nuclear antigen （PCNA） positive expression in suture cells around maxilla, and explore the improvement of suture expansion on the growth and development of maxilla.
METHODS：Tbe experiment was accomplished at the Laboratory of Oral and Maxillofacial Surgery, Stomatology College of the Fourth Military Medical University of Chinese PLA between March and September 2005. Twelve mongrel dogs of 15 weeks old were randomly assigned into four groups： one-week experimental group （n=4）, one-week control group （n=2）, four-week experimental group （n=4） and four-week control group （n=2）. The dogs of experimental groups were fitted with distraction devices, and traction hook was educed from palatine foramen, with rubber ring as connection between device and hook.Forwasd elastic traction was exerted respectively for I week and 4 weeks at 600 g force.All the dogs were executed at the end of force application, then transverse palatine suture （TPS）, premaxilla-maxillary suture （PMS）, zygomticomaxillary suture （ZMS） and zygomaticotemperal suture （ZTS） were harvested. PCDA monoclonal antibody was stained with immunohistochemical technique, while the distribution and amount of proliferating cells in sutures were detected and analyzed statistically. RESULTS：There were 12 dogs surviving until prearranged observation period. ①More PCNA-positive expressions were observed in cells of TPS and ZMS in one-week experimental group than in one-week control group （One-week experimental group： TPS： 48.69±5.42, ZMS： 46.44±3.72; Oneweek control group： TPS： 34.00±3.26, ZMS： 33.75±5.58, P〈0.05）.②And PCNA-positive expressions were higher in cells of all these sutures in fourweek experimental group than in four-week control group and one-week experimental group （Four-week experimental group： PMS： 70.78±3.58, TPS： 69.9±2.62, ZMS： 68.56±4.15, ZTS： 64.25±2.82; Four-week control group： PMS： 32.95±3.24, TPS： 34.83±5.68, ZMS： 34.26±3.37, ZTS： 33.46±3.47; One-week experimental group： PMS： 38.97±4.25, TPS： 48.69±5.42, ZMS： 46.44±3.72, ZTS： 36.06±4.34, P〈0.05）.
CONCLUSION：Significantly increased proliferating cells and their activities are observed in maxilla after suture expansion, which implies suture expansion can improve the growth and development of maxilla.
出处
《中国临床康复》
CSCD
北大核心
2006年第29期101-103,共3页
Chinese Journal of Clinical Rehabilitation
