摘要
目的:观察生理电场干预对大鼠主动脉血管平滑肌细胞迁移的影响。方法:实验于2002-07/2003-12在解放军第三军医大学西南医院中心实验室进行。取Wistar大鼠47只,颈离断法处死大鼠,体外培养主动脉血管平滑肌细胞。将细胞根据培养基分为DMEM组和DMEM+体积分数为0.1的胎牛血清组,分别给予0,50,100,150,200,250mV/mm生理电场干预6h,显微成像及图像分析技术记录分析血管平滑肌细胞迁移。结果:①在DMEM组,细胞在250mV/mm电场中没有定向迁移,电场干预没有明显提高细胞迁移速度。②在DMEM+体积分数为0.1的胎牛血清组,血管平滑肌细胞在电场作用下显示明显的趋化反应。在100～250mV/mm,细胞向阴极迁移。在150mV/mm电场中,单个细胞和细胞团定向迁移的速度没有明显差异[(12.79±0.77),(14.21±0.45)μm/h,P>0.05]。结论:生理电场诱导血管平滑肌细胞向阴极定向迁移,电场的作用依赖于电场强度和血清成分。
AIM： To investigate the effect of physiological electric field on the migration of cultured vascular smooth muscle cells （VSMC） of arterial aorta of rats.
METHODS： The experiment was eonducted at the Central Laboratory, Southwest Hospital, Third Military Medical University of Chinesl PLA from July 2002 to December 2003. A total of 47 Wistar rats were selected and killed by breaking their necks. VSMC of rats was cultured in vitro. The cells were assigned into DMEM group and DMEM＋fetal bovine serum with the 0.1 volume fraction group, and then were exposed to phys!ologieal electric fields of 0, 50, 100, 150, 200 and 250 mV/mm for 6 hours. The migration of VSMC was recorded and analyzed with micro-imaging andimage analysis. RESULTS： ①In DMEM group, there was no oriented migration in 250 mV/mm electric field. The intervention of electric field did not significantly elevate the speed of migration. ②ln the DMEM＋fetal bovine serum with the 0.1 volume fraction group, there was significant chemotactic response of VSMC in the effect of electric field. The cells migrated toward negative pole in 100-250 mY/ram. In 150 mV/mm, there was insignificant difference in migration speed of single cell and cell mass [（ 12.79±0.77）, （ 14.21 ±0.45 ）μm/h ,P〉0.05].
CONCLUSION： Physiological electric field orients VSMC migration toward negative pole. The migration effect of electric field is dependent on strength of electric field and serum.
出处
《中国临床康复》
CSCD
北大核心
2006年第29期80-82,i0003,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(30170236)~~
