摘要
目的:探讨在SCID小鼠体内移植人免疫细胞,建立人源化SCID小鼠模型及其特性鉴定。方法:SCID小鼠腹腔注射环磷酰胺(CTX)抑制骨髓造血,连续4天后,通过腹腔注射移植人外周血单个核细胞(PBMC)。4、8和12周后分别取小鼠外周血、脾脏、肝脏。荧光显微镜下观察SCID小鼠外周血中人CD3+、CD19+细胞;流式细胞仪测定全血中人CD3+、CD19+细胞百分率;免疫组织化学分析SCID小鼠肝脏和脾脏中人CD3+、CD19+细胞;ELISA检测SCID小鼠血清中人免疫球蛋白含量。结果:(1)SCID小鼠移植人外周血单个核细胞4、8和12周后在小鼠外周血中通过荧光显微镜下可观察到人CD3+、CD19+细胞,4周后流式细胞仪测得小鼠外周血单个核细胞中人CD19+、CD3+细胞百分率分别为10·6%、31·7%;(2)免疫组织化学结果显示在小鼠脾脏中存在人CD3+、CD19+细胞;(3)移植人外周血单个核细胞4、8和12周后ELISA测得小鼠血清中人免疫球蛋白的含量分别为390、1100和1040μg/ml。结论:成功地在SCID小鼠体内建立了人免疫系统。
Objective：To establish and identify humanized-SCID mouse model（hu-SCID）. Methods：SCID mouse was treated by CTX to inhibit the bemocytopoiesis. With successive 4-day injection, human peripheral blood mononuclear cells（PBMC） were engrafted into SCID mouse through intraperitoneal injection. After 4, 8 and 12 weeks of engraftment, peripheral blood, spleen and fiver tissues of engrafted SCID mouse were harvested. Human CD3^＋ , CD19^＋ cells in peripheral blood were analyzed by inflorescence micrescopy and FCM, human CD3 ^＋ , CD19 ^＋ ceils in spleen and liver tissues were observed by immune histochemistry, and human IgG level in SCID mouse serum was measured by ELISA. Results：After engraftment of 4, 8 and 12 weeks, human CD3^＋ , CD19^＋ cells in SCID peripheral blood were identified by inflorescence microscopy and the percents were 31% and 10% respectively by FCM analysis. And these cells could be evidenced after 12 weeks later. Through immune histochemlstry human CD3 ^＋ 、CD19 ^＋ cells were detected in mouse spleen but not in fiver tissue. Furthermore the titer of human IgG in mouse serum was 390,1 100 and 1 040 μg/ml at each time point respectively. Conclusion：Our experimental results demonstrated that a bona fide humanized SCID model was established.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第5期459-462,共4页
Chinese Journal of Immunology
基金
国家自然基金重点项目(30330540)
江苏省临床免疫学重点实验室基金资助项目
