摘要
目的了解不同硬腭裸露骨面面积与上颌骨及牙弓生长发育之间的关系及相关机制,为设计与选择合理的腭裂修复术式提供实验依据。方法选取3周龄SD雄性大鼠48只,随机分为对照组和3个实验组,每组12只。3组实验组大鼠分别被切去硬腭1/4、2/4和3/4面积大小的粘骨膜瓣,大鼠术后每周同期交替注射荧光标记物(对照组同期注射),每2周随机处死每组标记动物3只,解剖出上颌骨,制备成硬组织切片,测量上颌骨和牙弓宽度,观察荧光标记的差异。结果实验表明:①当裸露面积小于硬腭面积1/4时,上颌骨新骨形成和组织学变化不大,并且可获得接近正常的上颌骨和牙弓发育。②随着硬腭裸露面积的增加,上颌骨及牙弓生长发育受限越严重,牙齿内倾更加明显,组间存在着统计学差异。③裸露面积越大,对硬腭新骨形成和腭中缝的结构影响越大,表现为荧光标记线越不规则,标记间距变窄,当裸露面积达硬腭面积的3/4时,甚至产生弥散的荧光标记(即骨坏死)和腭中缝结构的丧失。结论硬腭裸露面积与上颌骨及牙弓发育之间的关系密切,裸露面积越大,上颌骨及牙弓发育受限越严重。
Objective To explore the relationship between the development of maxilla and dental arch and the size of denuded hard palate. Thus to get experimental proof for surgeons to choose a optimal surgical way for the treatment of cleft palate. Methods 48 three-week old SD male rats were divided into a control group and 3 experimental groups randomly. In the three experimental groups, different areas of bilateral palatal mucoperiosteum were excised respectively. Then the animals were injected with fluorescent labeling alternately by week. Biweekly, three animals in each group were sacrificed and the maxillae were dissected out and then measured the width of the maxilla and the dental arch, and investigated the difference of bone labelling. Results ①Whcn the area was less than one quarter area of the palate, there was no obvious change of new bone formation, and better growth of maxilla and dental arch can be acquired. ②With the increasing area of denuded bone on the palate, the maxilla and dental arch retardation was more severe and palatal inclination of tooth was more obvious. ③The larger the area of bone denudation, the more negative influence to new bone forming and sutural structure, represented by more irregular bone labelling. As the denudation area was beyond three quarters of the hard palate, bone labelling was disperse and sutural structure lost. Conclusion There is affinity between the area of denuded bone on palate and the growth of maxilla and dental arch. The bigger the denudation area is, the more severe the maxillary and dental arch constriction is.
出处
《国际口腔医学杂志》
CAS
2006年第4期326-329,250,共5页
International Journal of Stomatology
基金
教育部高等学校优秀青年教师教学和科研奖励基金资助(1999)
关键词
裸露骨面
上颌骨发育
牙弓发育
三色荧光标记
bone denudation
maxilla growth
dental arch growth
triple fluorescent labeling
