摘要
目的探讨氨基胍(AG)对大鼠脑缺血再灌注后的血脑屏障的影响及对再灌注损伤的保护作用。方法采用线栓法建立鼠大脑中动脉局灶缺血再灌注模型。于缺血2 h再灌注22 h,测定脑梗死体积,并用伊文思蓝染色荧光显微镜观察血脑屏障破坏的程度,HE染色观察中性粒细胞浸润变化。结果再灌注22 h后,AG治疗组脑梗死体积减小,脑缺血区血脑屏障破坏程度和中性粒细胞浸润程度明显减轻(P<0.05)。结论AG对脑缺血再灌注具有确切的脑保护作用,并能减轻脑缺血再灌注对血脑屏障的破坏程度和炎症细胞浸润程度。
Objective To evaluate the therapeutic effects of aminoguanidine（AG） on cerebral ischemia-reperfusion damage in rats. Methods The intravascular thread models with 2 h of occlusion and 22 h of reperfusion were made in the rats. The brain infarction size and the degree of blood brain barrier （BBB） disruption in the ischemic regions were evaluated by staining with 2, 3, 5-triphenyl tetrazolium chloride and observing with Evans blue fluorescence microscope. HE staining was utilized for observing neutrophil infiltration. Results The brain infarction volume, the area of BBB disruption and the degree of neutrophil infiltration were dramatically decreased in the treatment group as compared to the control group（P 〈 0.05）. Conclusion AG has definite protective effect on cerebral ischemia-reperfusion injury. It can also attenuate BBB disruption and neutrophil infiltration.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2006年第7期755-757,共3页
Journal of Shanghai Jiao tong University:Medical Science
关键词
脑缺血
再灌注损伤
氨基胍
血脑屏障
cerebral ischemia damage
reperfusion damage
aminoguanidine
blood brain barrier
