摘要
目的研究Aβ对大鼠记忆、空间定向能力的影响及尼古丁的干预作用。方法将Aβ1-40注入大鼠双侧基底前脑中,建立大鼠基底前脑胆碱能神经元损伤模型,观察大鼠学习和空间定向能力的变化,与此同时我们通过腹腔注射烟碱受体激动剂尼古丁对此模型进行干预。结果Aβ注射组大鼠在定位航行试验中,平均逃避潜伏期明显延长,在空间探索试验中跨越原平台位置的次数明显减少,将大鼠在第5天寻找平台的方式进行分类记录,有显著差异(P<0.05)。两周组的尼古丁干预大鼠能够部分地改善由Aβ注射引起的学习记忆损伤(P<0.05,P<0.01),四周组的大鼠未显示出有统计学意义的记忆和空间定向能力的减退(P>0.05)。结论短期(2周)Aβ损伤大鼠学习记忆能力明显下降,从行为学上来评价,该模型是成功的;尼古丁在体内能够部分地改善由Aβ注射引起的学习记忆损伤。四周组的大鼠未显示出有统计学意义的记忆和空间定向能力的减退,有可能是因为Aβ随着时间的延长逐渐被代谢。
Objective To establish rat models with Alzheimer＇s disease （AD） induced by β-amyloid, and to ohserve the effects of nicotine on learning and memorizing ability of the rats. jected into the bilateral basal forebrain of the rats. Learning and memorizing Morris water maze test. The effects of nicotine on learning and memorizing peptide were observed. Results The learning and memorizing ability of Methods Aggregated Aβ1-40 was inability of the rats were inspected through ability of the rats induced by β-amyloid AD animal models injected aggregated Aβ1-40 for 2 weeks decreased （ P 〈 0.05 ）. Nicotine partly improved the learning and memorizing ability of the models （ P 〈 0.05, P 〈 0.01 ）. Conclusion Microinjection of aggregated Aβ1-40 into bilateral basal forebrain of rats may induce memory disorder and neuronal damage. Nicotine can resist nerve poison of β-amyloid peptide, and the mechanism may be relevant to its function of purging brain free radicals and increasing activities of antioxidase.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2006年第7期719-724,共6页
Journal of Shanghai Jiao tong University:Medical Science
基金
上海市自然科学基金(01ZB14050)
上海市引进海外高层次留学人员专项基金(20040115)
上海市重点基金(024119037)资助项目
关键词
Β-淀粉样蛋白
老年性痴呆
学习记忆
尼古丁
水迷宫试验
β-amyloid peptide
Alzheimer＇ s disease
learning and memorizing
nicotine
Morris water maze test
