摘要
目的探讨沙尘暴细颗粒物(PM2.5)对人外周血淋巴细胞的遗传损伤。方法用采集自内蒙古包头市和甘肃武威市的沙尘暴和正常天气PM2.5不同浓度(0、33、100、300μg/ml)提取物染毒人外周血淋巴细胞,用染色体畸变试验和胞质阻断法进行畸变率和微核率统计。结果包头、武威沙尘暴和正常天气PM2.5提取物的100、300μg/ml剂量组人血淋巴细胞微核率均高于其对照组(均P<0.01)。包头、武威沙尘暴300μg/ml剂量组和正常天气PM2.5提取物的100、300μg/ml剂量组人血淋巴细胞核分裂指数均低于其对照组(P<0.05或P<0.01);染毒剂量与微核率呈正相关,与核分裂指数成负相关。不同浓度沙尘暴PM2.5提取物染毒能引起人外周血淋巴细胞染色体结构畸变,主要表现为染色体及染色单体断裂、缺失、成环、形成双着丝粒(染色体桥)、断片等,同时也明显增加了淋巴细胞染色体畸变率,且存在剂量-反应关系(P<0.01)。结论沙尘暴PM2.5能引起淋巴细胞遗传损伤,其遗传毒性主要与染毒剂量有关。
Objective To investigate the genetic damage of dust storm fine particles on human blood lymphocytes. Methods The chromosomal aberration test and cytochalasin B blocked test were used to investigate the effect in vitro of dust storm fine particles （PM25） （0, 33, 100, 300 μg/ml）collected in Baotou and Wuwei cities on human lymphocytes. Results In both dust storm and normal ambient air fine particles treated cells revealed an increase in the chromosomal aberration level and micronuclei frequency （MNF）. The chromosomal aberration （CA）were characterized as chromatid break, chromosome break, acentric fragment, dicentric chromosome and gaps. With the increase of treatment concentrations the aberration level and MNF increased and the mitotic index（MI） and the nuclear division index （NDI） values declined in a dose-response manner（P〈0.01）. In the same concentration, the values of treated with normal weather particles were higher than those of treated with dust storm, but the treatments of normal ambient air PM25 from Baotou City were significant higher than those of dust storm （P〈0.01）,and the treatments of dust storm and normal ambient air PM25 from Wuwei City were not significant different （P〉0.05）. The treatments of normal ambient air PM25 from Baotou City were significant higher than those of Wuwei City, but the treatments of dust storm PM25 were not significant different between the cities. Conclusion Dust storm PM25 from Baotou City and Wuwei City may cause human lymphocytes genetic damage and its genetic toxicity is related to the dose.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第4期291-293,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30230310)
山西省自然科学基金资助项目(20031092)
关键词
空气污染
沙尘暴
细颗粒物
染色体畸变
Air pullotion
Dust storm
Fine particles
Chromosome aberrations
