摘要
利用高效液相色谱法对邻硝基甲苯和对硝基甲苯2种同分异构体的分离作了系统的研究.选择十八烷基键合相色谱柱COSMOSIL5C_(18)-AR-Ⅱ(6.0mm×250mm),甲醇作为样品溶剂,甲醇和水的混合物作为流动相.确定了分离的最佳色谱条件为室温、V(甲醇)∶V(水)=70∶30、检测波长278nm、流动相流速1.0mL/min.回收率验证结果显示,准确度和灵敏度均符合分离要求.
In this paper, the separation of p-nitrotoluene and o-nitrotoluene was studied comprehensively by high performance liquid chromatography. By comparison, C18 bonded-phase chromatography column of COSMOSIL 5C18-AR- Ⅱ（6.0 mm× 250 mm）was selected, methanol as solvent of specimen and mixture of methanol and water as mobile phase. The best chromatogryphy conditions are： room temperature, V（methanol） ： V（ water） = 70：30, detected wavelength is 278 nm and velocity of flow 1.0 mL/min. In addition, the method was validated by recoveries experiment. The results show this method is reproducible and sensitive enough for separation of p-nitrotoluene and o-nitrotoluene.
出处
《天津大学学报》
EI
CAS
CSCD
北大核心
2006年第7期885-888,共4页
Journal of Tianjin University(Science and Technology)
关键词
高效液相色谱
邻硝基甲苯
对硝基甲苯
同分异构体
high performance liquid chromatography
p-nitrotoluene
o-nitrotoluene
isomers
