摘要
目的探讨人类骨形成蛋白2(hBMP-2)基因修饰的组织工程化骨修复骨质疏松症者骨缺损的可行性及方法。方法24只6个月龄雌性SD大鼠建立去势模型,按体重编号,随机分组。3个月后实验组骨髓间质干细胞(BMSC)转染hBMP-2质粒,对照组未作干预,在体外构建自体细胞组织工程化骨,植入下颌骨缺损区。结果术后4周时实验组有新生骨质形成,8周时成熟骨基质形成;对照组新生骨质数量明显少于实验组,且材料边缘及中央处有脂肪样结构形成。结论hBMP-2基因修饰的组织工程化骨可用于骨质疏松症者骨缺损的治疗。
Objective To investigate the feasibility of repairing bone defect with methods of tissueengineering and human bone morphogenetic protein-2 （hBMP-2） gene transfection in osteoporotic rats. Methods Twenty-four 6-month-old female Sprague-Dawley rats underwent ovariectomy, while 8 rats received sham-operations. Three months later, bone mesenchymal stem cells （BMSC） harvested from osteoporotic rats were divided into two groups randomly. Experimental group were transfected by recombinant plasmid carrying hBMP-2 gene, and control group leaved untreated. All BMSC were seeded into coralhydroxyapatite scaffolds. Then the cell/scaffold constructs were implanted into the defect site createdin the ramus of mandible of osteporotic rats respectively. Results Positive results were confirmed by immunohistochemistry and in situ hybridization in experimental group. New bone formation was found at the margin of the defect treated with the BMSC modified by hBMP-2 gene transfer at 4 weeks after implantation and appeared mature 8 weeks after the treatment. However , the amount of newly formed bone was much less and there was some adipose tissue at defect margins 8 weeks after implantation in control group. Conclusions The results of this experiment indicate that BMSC-mediated rhBMP-2 gene therapy in conjunction with bone tissue engineering may allow for successful treatment of large bone defects in osteoporosis rats.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2006年第7期430-431,共2页
Chinese Journal of Stomatology
基金
教育部高校优秀青年教师教学科研奖励计划基金资助项目(2003682)
四川省应用基础研究基金资助项目(02SY029-116)
关键词
骨质疏松
骨移植
基因疗法
Steoporosis
Bone transplantation
Gene therapy
