摘要
以ＣＡＳ－２００型图象细胞仪（ＩＣＭ）对９９例肝细胞癌（ＨＣＣ）行ＤＮＡ定量分析。结果：包膜完整、包膜不完整、和无包膜三组ＨＣＣ之间，增殖指数、细胞核面积、二倍体肿瘤比例均有显著的统计学差异（Ｐ＜０．０１，Ｐ＜０．０５，Ｐ＜０．０５）。在无门静脉癌栓（ＴＴＰＶ）、合并镜下ＴＴＰＶ、和合并肉眼ＴＴＰＶ的三组ＨＣＣ间，增殖指数和ＤＮＡ指数统计学差异显著（Ｐ＜０．０１，Ｐ＜０．０５）。合并肝内播散组ＨＣＣ，增殖指数及超五倍体细胞比例分别显著高于无肝内播散组（Ｐ＜０．０５，Ｐ＜０．０１）。然而组间比较表明，包膜不完整组与无包膜组之间，合并镜下ＴＴＰＶ组与合并肉眼ＴＴＰＶ组之间，ＩＣＭ的各项参数均无统计学差异。结论：①随着ＤＮＡ增殖水平的上升，ＨＣＣ细胞获得了更强的侵袭性和转移能力；②以ＩＣＭ行ＤＮＡ定量分析对于揭示ＨＣＣ是否具有更强侵袭性和转移潜能有明显价值，其中增殖指数检测具有效高的敏感性；③进一步研究ＩＣＭ的各项参数对ＨＣＣ预后的价值是有意义的。
PURPOSE The invasiveness and metastasis is the key cause of poor prognosis of hepatocellular carcinoma(HCC). It has been reported that DNA content analysis revealed the proliferating activity in HCC. To determine the correlation between invasiveness and metastasis with DNA content in HCC, DNA content analysis was performed.METHODS A total of 99 paraffin-embedded materials of HCC were studied with CAS-200 DNA image cytometry(ICM). Tumor capsule formation,tumor thrombi in portal vein(TTPV) and daughter nodule formation were regarded as manifestations of invasiveness and metastasis in HCC.Analysis of variance was performed using a computerized statistical package(Epilog software). RESULTS There were significantly statistic differences within the well capsulated group, the poorly capsulated group and the group without capsule in proliferating index(PI)(P<0.01), nuclear area(NA)(P<0.05) and diploid(2C)(P<0.05). The statistic difference in PI(P<0.01), as well as in DNA index(DI)(P<0.05), was also revealed within the group accompanied by macroscopic TTPV, the group accompanied by microscopic TTPV and the group without TTPV. PI and pentaploidy exceeding cell rate (5CER) were significantly higher in group with hepatic daughter nodules than those without(P<0.05,P<0.01). CONCLUSIONS ①HCC cells obtained stronger invasive and metastatic capability once DNA proliferating process increased, ②Using DNA image cytometry, especially detecting PI which showed the highest sensitivity in ICM, has noticeable value in revealing the potency of invasiveness and metastasis in HCC; and ③Further study focusing on predicting the prognosis of HCC using parameters of ICM might be valuable.
出处
《上海医科大学学报》
CSCD
1996年第4期257-260,共4页
Journal of Fudan University(Medical Science)
