摘要
用细菌内毒素建立豚鼠的牙髓炎模型，借助体外培养的Ｌ９２９细胞，在培养液中加入正常和炎症的牙髓组织上清液，采用成纤维细胞增殖法测定正常和炎症牙髓组织中白细胞介素１（ＩＬ１）的活性。结果发现正常牙髓组织无ＩＬ１的活性，炎症牙髓组织产生明显的ＩＬ１活性，并随内毒素诱导时间的延长而增强。ＩＬ１为牙髓炎的主要介质之一，介导了牙髓炎的发生和发展。
The authors established guinea pig pulp inflamation model with large intestine bacillus lipopoly saccharide (Ecoli O 111 :B 4, LPS). In order to detect the activity of IL 1 in healthy and inflamed pulp surpernates, the authors used rat lung fibroblasts (FB, L 929 cells) as response cells. The results of the experiment were as follows: healthy pulp had no activity of IL 1, inflamed pulp marked activity of IL 1, the activity of IL 1 increased when inducing time increased, the activity of IL 1 was time dependent. In conclusion, the authors have demonstrate that IL 1 is a primaty mediator of inflamed pulp which mediates occuring and developing of pulp inflamation.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1996年第3期177-179,共3页
West China Journal of Stomatology
关键词
细菌内毒素
牙髓炎
白细胞介素1
L929细胞
bacteria lipopoly saccharide\ \ pulp inflamation\ \ interleukin 1(IL 1)\ \ rat lung fibroblasts(FB, L 929 cells)
