摘要
运用免疫组织化学技术,以P53基因蛋白和增殖细胞核抗原(PCNA)表达为指标,探讨了人肺癌组织中的P53基因突变与细胞增殖的关系。结果表明:癌组织中的P53基因突变与肿瘤细胞的增殖活性呈正相关。表明突变型P53有促进细胞生长的作用,为阐明P53基因在细胞生长调控中的作用增添了实验依据。
In this study,we used an immunohistochemical technique to observe the expression of P53 protein and proliferating cell nuclear antigen(PCNA)for the probe of the relationship between the mutation of P53 gene and cell proliferation in human lung cancer.It was found that the positive correlation was obvious between the mutation of P53 gene and tumor cell proliferative activity in the cancer tissue.The research result reveals the promoting action of mutant P53 gene in tumor cell growth and provides experimental evidence for explanation on the function of P53 gene in the adjustment of cell growth.
作者
喻伦银
刘铭球
邹祖玉
江曼
张正彬
Yu Lunyin;Liu Mingqiu;Zou Zuyu(Department of Pathology,Basic Medical College,Hubei Medical University,Wuhan,430071,China)
基金
湖北省科委重点科技项目资助
关键词
肺肿瘤
遗传学
P53
基因突变
PCNA
lung neoplasms/GE
suppression
genetic
cell division
histological technics
antinuclear factors
