摘要
目的观察多次使用氯胺酮对学习记忆的影响及剂量效应关系。方法30只SD大鼠随机分为高剂量组、低剂量组和对照组,高、低剂量组大鼠分别予以氯胺酮50 mg/kg和10 mg/kg腹腔注射麻醉,每日1次,连续7日。对照组予以等容生理盐水。用Morris水迷宫测试各组大鼠寻找隐匿台的逃避潜伏期和空间搜索能力,用电镜观察海马神经元超微结构。结果高剂量组逃避潜伏期显著延长(P<0.01),并且空间搜索能力明显降低(P<0.01),对照组与低剂量组之间有关指标无显著性差异。电镜观察显示高剂量组海马神经元有明显变性。结论高剂量氯胺酮多次麻醉对学习记忆有损害作用。
Aim To study the effectson the learning and memory behavior of rats after Using ketamine（Ket） Anesthesia repeatedly. Methods 30 rats were randomly divided into 3 groups（50 mg/kgKet,10 mg/kgKet and control groups）. The two treating groups rats were respectively anesthetized with 50 mg/kg and 10 mg/kg ketamine ip qd for a week and the control rats were given equal volume of 0. 9 % saline ip. The Morris water maze was used to test the ability of learning and spatial localization on rats. At the end of experiment,the hippocampus was removed for electron microscopic examination. Results In 50 mg/kg Ket group,the escape latency was significantly longer（ P 〈 0.01 ） and the ability of spatial localization was lower than the other groups（ P 〈 0.01 ）. But there were no significant difference of the indexes of the learning and memory behavior between groups of 10 mg/kg Ket and the control. Electron microscopy showed that some obviously degenerative changes occurred in hippocampal neurons of rats treated with 50 mg/kg Ket. Conclusion Repeated anesthesia with a higher dose of ketamine may affect the ability of learning and memory.
出处
《安徽医药》
CAS
2006年第7期490-491,共2页
Anhui Medical and Pharmaceutical Journal
