摘要
目的用乳酸脱氢酶(LDH)法检测恶性疟原虫融合蛋白(PfCP-2.9)免疫兔血清体外抑制恶性疟原虫生长的效果。方法将恶性疟原虫起始培养物的原虫率稀释为(0.4±0.1)%,红细胞压积为2%,使用不同浓度恶性疟原虫融合抗原PfCP-2.9免疫兔血清进行恶性疟原虫体外生长抑制试验,40～42h后分别用LDH检测法和传统镜检计数法检测原虫生长抑制率。结果PfCP-2.9免疫兔血清在体外对恶性疟原虫有较强的抑制作用。LDH测定法与传统镜检法测得体外恶性疟原虫抑制率相近(分别为97%和100%),两者差异无统计学意义(P>0.05)。结论LDH法检测恶性疟原虫体外抑制试验是一种简单、可行的实验方法。
Objective To investigate the in vitro inhibition effect of the rabbit serum immunized with P.falciparum chimeric protein on the parasites by lactate dehydrogenase （LDH） method. Methods Sera of rabbits immunized with P.falciparum chimeric protein were used as test sample, with an initial parasitemia of （0.4±0.1）% and 2% hematocrit suspension. After 40-42 h of cultivation, LDH assay and a traditional microscopical method were applied to obtain the parasite inhibition rate and infection rate of erythrocytes. Results Sera of rabbits immunized with the protein showed a strong in vitro inhibition effect on P.falciparum. There was no significant difference of inhibition rates detected by the two methods （97% and 100% respectively） （P〉0.05）. Conclusion The LDH method applied in the in vitro inhibition assay for P. falciparum seems simple, reliable and has generated a satisfactory result.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第3期223-225,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
恶性疟原虫
体外抑制试验
乳酸脱氢酶
Plasmodium falciparum
In vitro inhibition
Lactate dehydrogenase
