摘要
目的观察核糖体蛋白L6(RPL6)表达的改变对白血病耐药性的作用。方法通过RT-PCR方法获得RPL6cDNA序列,用真核表达载体pcDNA3.1(+)分别构建正向插入和反向插入的RPL6cDNA重组质粒。以脂质体将正义RPL6cDNA真核表达质粒转染K562细胞,将反义RPL6cDNA真核表达质粒转染K562/A02细胞,以MTT观察其对化疗药物耐药性的作用。结果转染正义RPL6cDNA真核表达质粒后,K562细胞对阿霉素的耐药性比未转染的K562细胞增强3.25倍,转染反义RPL6cDNA真核表达质粒后,K562/A02细胞对阿霉素的耐药性比未转染的K562/A02细胞降低62%。结论RPL6基因过表达在K562/A02细胞耐药性的形成中起重要作用。
Objective To investigate the effect of ribosomal protein L6 （RPL6） gene on the resistance of leukemia cell to antitumor drugs. Methods RPL6 cDNA was obtained by RT-PCR ,Both sense and antisense cDNA reeombinants of RPL6-encoding gene were constructed with pcDNA3.1 （ ＋ ） expression vector ,then the sense RPL6 cDNA recombinant was transfected into K562 cells while the antisense one into K562/A02 cells by liposomal reagent. The chemosensitivity of cells was detected by MTT assay. Resuits Internal control RT-PCR showed higher expression of RPL6 in K562/A02 than in K562; Sense-transfected K562 cells were 3.25 times more resistant to adriamycin than control cells did, whereas resistant to adriamycin of antisense-transfected K562/A02 cells decreased by 62 % than that of control cells;Conclusion RPL6 gene plays an important role in the development of drug resistance in K562/A02 cells.
出处
《实用癌症杂志》
2006年第2期117-119,共3页
The Practical Journal of Cancer
基金
湖南省卫生厅科研基金资助(B2004-090)
