摘要
为了进一步提高肾移植的水平，采用顺序特异引物聚合酶链反应（ＰＣＲ－ＳＳＰ）方法用于２７６例肾移植供受者ＨＬＡ－ＤＲ基因配型，以探讨其临床可行性和实用性．结果显示：２７６例供受者３０８份样本，ＰＣＲ－ＳＳＰ分型均获成功，检测时间５小时内。供受者ＤＲ等位基因频率分布大致相同，无显著性差异。已行移植的１３５例受者分为配型选择组５１例和随机选择组８４例。配型组０～１个位点错配率５４．９％，明显高于随机组的２８．６％，而２个位点错配率为４５．１％，明显低于随机组的７１．４％，具有非常显著性差异（Ｐ＜０．０１）。研究表明，ＰＣＲ－ＳＳＰ快速ＨＬＡ－ＤＲ基因分型技术选择供受者适合于临床应用并具有很强的实用性。
HLA-DR alleles was genotyped for 276 donor-recipients of cadaveric renal transplantation by polymerase chain reaction with sequence-specific primers (PCR-SSP).308 samples of 276 individuals could be thus accurately typed The matching usually took 5 hours.The gene frequencies of DR alleles were not significantly different between the donor and the recipient. The grafted patients consisted of 51 matching group and 84 random group patients.0￣1 DR dismatch was obviously increased in the matching group( 54.9%)as compared with the random group(28. 2% ) while 2 DR dismatch was significantly lower(45.1% )than the random group(71.4%). The difference has been conspicuous(P<0.01).Genotyping for HLA-DR by PCR SSP was recommended for clinical practice in organ transplantation.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1996年第12期707-709,共3页
Chinese Journal of Urology
基金
国家自然科学基金
上海市医学领先专业科研基金
