摘要
目的动态观测高氧肺损伤动物模型中(IFN-γ)和转化生长因子β1(TGF-β1)的变化规律,探讨高氧肺损伤纤维化的发病机制。方法取2周左右的Wistar大鼠32只,随机分为空气组和高氧暴露3、7、14 d组,采用逆转录聚合酶链反应(RT-PCR)和免疫组织化学染色观察IFN-γ和TGF-β1在空气和高氧暴露3、7、14 d组肺组织中的分布和表达。结果IFN-γmRNA在高氧3 d达高峰水平,高氧7 d后开始下降,高氧14 d下降更为明显,与空气组比较P分别<0.05,>0.05,>0.05;TGF-β1mRNA的表达在高氧3、7、14 d均明显高于空气组(P均<0.05);高氧3 d IFN-γ/TGF-β1比值显著增高(P<0.05),高氧7 d开始下降(P>0.05),高氧14 d明显下降(P<0.05)。免疫组织化学染色也显示了基本一致的动态变化趋势。结论高氧肺损伤早期,IFN-γ的升高促进了高氧肺损伤炎症的发展;高氧肺损伤中晚期IFN-γ和TGF-β1的失衡可能是促进高氧肺损伤纤维化发展的原因。
Objective To observe the dynamic changes of interferon gamma（ IFN γ） and transforming growth factor batal （TGF β1 ） in animal model of hyperoxia- induced lung injury, and to explore the mechanism of fibrosis. Methods Thirty- two juvenile Wistar rats were randomly divided into room - air group and hyperoxia group, the hyperoxia group were further divided into 3 subgroups of hyperoxia - exposure for 3, 7 and 14 days. The mRNA levels of IFN - γ and TGF - β1 in the lung were measured by reverse - transcription polymerase chain reaction （RT - PCR）. The expressions of IFN - γ and TGF β1 protein were measured by ning. Results The level of IFN - γ mRNA of groups under hyperxia - exposure for 3, 7 and 14 days were higher than that of control group. The expression of IFN- γ mRNA notably elevated and was up to its peak （P 〈 0.05）at the 3rd day, then gradually declined from the 7th day and obviously more after the 14th day. The levels of TGF β1 mRNA of the groups under hyperxia - exposure for 3, 7 and 14 days were significantly higher than that of control group（P〈0.05）. The ratio of IFN - γ and TGF β1 elevated significantly at the 3rd day, began to decrease from the 7th day and strikingly declined at the 14th day. Meanwhile immunohistochemical staining showed the similar changes. Conclusions At the early period of hyperoxia - induced lung injury, the increased IFN -γ promotes the progress of lung inflammation; while at the late stage, the disequilibrium between IFN-γ and TGF- β1 may attribute to acceleration of the development of lung fibrosis.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第12期735-736,757,共3页
Journal of Applied Clinical Pediatrics
关键词
高压氧
Γ干扰素
转化生长因子Β1
hyperoxia
interferon gamma
transforming growth factor batal
