摘要
目的探讨高压氧对ANP胰腺腺泡细胞核转录因子NF-κB活性的影响。方法采用5%牛磺胆酸钠胰管逆行注射制备ANP动物模型。SD雄性大鼠40只,按随机分配原则分为ANP高压氧治疗组和ANP空白对照组。采用EMSA法检测胰腺腺泡细胞胞核NF-κB活性、Western-blot法检测胰腺腺泡细胞胞质IκBa活性及ELISA法检测血液IL-2、IL-6、IL-10、TNF-a及ICAM-1含量。结果高压氧在1、3、5及7h均可显著抑制ANP胰腺腺泡细胞胞核NF-κB活性[(24.47±5.39)μmol/Lvs(31.36±5.72)μmol/L、(28.58±4.51)μmol/Lvs(39.44±6.31)μmol/L、(26.47±4.13)μmol/Lvs(33.80±5.96)μmol/L及(20.38±3.79)μmol/Lvs(25.69±4.91)μmol/L](P<0.01);显著增强ANP胰腺腺泡细胞胞质IκBa活性[(8.94±1.43)μmol/Lvs(6.37±1.19)μmol/L、(7.83±1.72)μmol/Lvs(5.91±1.65)μmol/L、(8.31±1.54)μmol/Lvs(6.85±1.37)μmol/L及(8.62±1.12)μmol/Lvs(6.97±0.86)μmol/L](P<0.01);显著抑制血液中炎症细胞因子IL-2、IL-6、IL-10、TNF-a及ICAM-1活性(P<0.05)。结论高压氧可通过显著抑制ANP胰腺腺泡细胞NF-κB活性,增加胰腺腺泡细胞胞质IκBa活性的能力,减少血液中炎症细胞因子活性,从而抑制ANP引起的过度炎症反应。
[Objective] This study is to investigate the effect of hyperbaric oxygen（HO） on transcription factor nuclear-κB （NF-κB） activity of Pancreatic Acinar Cells in Acute Necrotizing Pancreatitis（ANP） of rats. [Methods] Fourty rats were randomly divided into two groups, namely group A： ANP（＋） HO treatment group （n =20） and ANP contrast group （n =20）. ANP was induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct. NF-κB activity in the cell nuclear and IκBa activity in the cell spasm of pancreatic acinar cells were measured by electrophoretic mobility shift assay （EMSA） and Western-blot respectively. The concentrations of inflammatory cytokines （IL-2, IL-6, IL-10, TNF-a and ICAM-1） of the blood were measured by ELISA. [Results] NF-κB activity of pancreatic acinar cells of ANP rats is strikingly inhibited （P 〈0.01） and IκBa activity is greatly increased （P 〈 0.01） by HO compared with the contrast group at 1, 3, 5 and 7 h respectively. The concentrations of inflammatory cytokines （IL-2, IL-6, IL-10, TNF-a and ICAM-1） in the blood of ANP rats remarkably decrease in group A compared with the group B at 1, 3, 5 and 7 h respectively （P 〈0.05）. [Conclusion] The results indicate that HO can significantly inhibit the NF-κB activity and increases IκBa activity of pancreatic acinar cells in experimental ANP of rats, and further reduces the production of inflammatory cytokines, alleviating the systemic inflainmatorv response.
出处
《中国医学工程》
2006年第3期230-233,共4页
China Medical Engineering
基金
湖南省自然科学基金资助项目(项目编号:02jjy3026)
