摘要
人外周血单个核白细胞低密度脂蛋白受体的ｍＲＮＡ为低丰度表达，不易检出，本文应用反转录－多聚酶链反应技术建立的低密度脂蛋白受体基因表达定量检测法灵敏、简捷、特异。为临床观察高胆固醇血症患者和冠心病易患人群的低密度脂蛋白受体表达情况，提供了方便必要的手段，初步应用结果表明，高胆固醇血症和冠心病患者的低密度脂蛋白受体ｍＲＮＡ水平明显降低。
Low density lipoprotein receptor(LDLR) mRNA in peripheral blood mononuclear cells(PBMC) belong to low abundance expression and is difficult to detect.This study found that using the reverse transcription(RT)-polymerase chain reaction(PCR)procedure for semiquantitative determinating expression levels of LDLR gene is a highly sensitive and specific,and gives a convinient and necessary method for clinical studying expression levels of LDLR gene in PBMC from patients with hypercholesterolemia and coronary heart disease.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第2期141-144,共4页
Chinese Journal of Arteriosclerosis
关键词
低密度脂蛋白
高胆固醇血症
冠心病
受体基因
Low density lipoprotein
Receptor
Gene expression
Peripheral blood mononuclear cells
Reverse transcription-polymerase chain reaction
