摘要
目的:观察不同浓度的过氧化氢(H2O2)对体外培养的正常人表皮黑素细胞增殖活性的影响。方法:建立正常人表皮黑素细胞体外培养体系,分别以0.05、0.1、0.5、1 mmol/L的H2O2作用于黑素细胞24 h,观察细胞形态学变化,并采用四甲基偶氮唑蓝(MTT)还原法检测细胞活力。结果:正常人表皮黑素细胞经H2O2处理后,细胞存活率降低,且呈浓度依赖性。结论:H2O2有抑制正常人表皮黑素细胞增殖的作用。
Objective：To study the effects of different concentrations of H2O2 on proliferation of human epidermal melanocytes. Methods： Human epidermal melanocytes were cultured for 24h with 0.05,0. 1,0. 5,1 mmol/L H2O2 respectively. The morphological change of melanocytes was observed under phase contrast microscopy, and MTT reduction assay was used to determine the melanocytes viability. Results： Comparing with the normal controls, H2O2 inhibited the melanocytes viability with a concentration-dependent manner. Conclusion： H2O2 could inhibit the proliferation of human epidermal melanocytes.
出处
《岭南皮肤性病科杂志》
2006年第2期82-83,86,共3页
Southern China Journal of Dermato-Venereology
基金
广东医学科学技术研究基金项目(项目编号:A2004144)
