摘要
为了进一步了解浙江宁海卫氏并殖吸虫(Paragonimus westermani)的遗传变异情况,对卫氏并殖吸虫不同种群进行ITS2序列分析研究。分别自浙江宁海小汀、宁海西溪(冷风洞)和宁海余山采集浙江华溪蟹,分离卫氏并殖吸虫囊蚴并进行ITS2序列PCR扩增分析。结果表明:浙江宁海三地卫氏并殖吸虫ITS2序列有363个碱基对,肺型肺吸虫和非肺型肺吸虫的ITS2序列完全相同;与泰国种群(AF159604,NakornNayok省)核酸同源性为97.5%,两地卫氏并殖吸虫遗传变异较大。浙江宁海卫氏并殖吸虫与日本、韩国等地的卫氏并殖吸虫的核酸同源性均较高,属亚洲东北组群。
In order to understand the genetic variation of Paragonimiasis westermani in Ninghai county, Zhejiang province, the metacecariae were sampled from Xiaoting, Xixi and Yushan village respectively for their ITS sequences analysis. Results indicated that all ITS of the three populations contained 363 base pairs nucleotide acid with the same sequence. The similarity of P. westermani ITS sequence was 97.5 % between the P. westermani populations in Zhejiang and the population from Nakorn Nayok province （AF159604） in Thailand, which appeared lower compared to those between the three P. westermani populations in Zhejiang and populations in Japan Korea and Taiwan province of China, so the P. westermani populations obtained in the three villages in Ninghai should belongs to Northeast Asia group.
出处
《寄生虫与医学昆虫学报》
CAS
2006年第2期77-80,共4页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家科技部中国泰国长期合作项目(17603J)
浙江省科技厅项目(2005F13023)
