摘要
目的:研究溃疡性结肠炎(UC)患者OX40分子的表达,以及CD4+OX40+T细胞的免疫表型和免疫学功能。方法:从活动性UC患者肠粘膜标本中分离和纯化粘膜固有层CD4+T细胞(LP-CD4+T)。用FACS多参数分析法测定OX40在不同的CD4+T细胞上的表达。用ELISABrdU(5-溴脱氧尿嘧啶)法测定LP-CD4+T细胞的增生程度以及不同刺激剂对它们增生程度的影响。结果:UC病变部位LP-CD4+T细胞表达OX40明显高于健康对照者外周血、UC患者外周血CD4+T细胞以及UC患者非病变部位CD4+T的表达。LP-CD4+OX40+T细胞表达淋巴细胞活化标记CD25、CD38、CD45RO和HLA-DR均明显高于LP-CD4+OX40-T细胞的表达(P<0.01)。抗OX40单抗可明显增强病变部位LP-CD4+T细胞的增生反应。相反,抗OX40L单抗则能明显抑制病变部位LP-CD4+T细胞的增生。结论:OX40在UC患者病变部位的LP-CD4+T细胞上表达明显增加,LP-CD4+OX40+T细胞是一类在原位被特异性抗原激活后扩增的T细胞,它们在UC的免疫病理机制中起重要作用。抗OX40L能够抑制其增生反应,可能是一种有效治疗UC的新方法。
To investigate the expression of OX40 on CD4^＋T cells in the patients with ulcerative colitis（UC） ,and analyze the phenotype and functional features of lamina propria（LP）-CD4^＋OX40^＋T cells from uc.nethods：The expression of OX40 molecule was measured by FACS.LP-CD4^＋T cells were cultured with different stimuli,and proliferation was assessed by ELISA BrdU.Results：The expression of OX40 was significantly higher on LP-CD4^＋T cells from inflammatory colons in UC patients.LP-CD4＋OX40^＋T cells expressed high levels of lymphocyte activation markers CD25,CD38,CD45RO and HLA-DR,which significantly higher than LPCD4^-T cells did respectively （all P〈0.01）.In vitro culture,APC induced proliferation response was further increased by anti-OX40 MoAb stimulation.But this proliferation response could be inhibited markedly by antiOX40L MoAb.Conclusion：OX40 is highly expressed on LP-CD4^＋T cells from inflammatory colons in patients with UC.LP-CD4^＋OX40^＋T cells are a subset of autoreactive T cells stimulated by specific antigen in situ.Anti- OX40L MoAb can inhibit the proliferation response of this T cells.These data indicate that OX40^＋T cells are involved in the immunopathological process in UC,and targeting OX40 is a new considerable strategy for the treatment of this disease.
出处
《中日友好医院学报》
2006年第3期137-140,共4页
Journal of China-Japan Friendship Hospital
