摘要
应用GUS基因标记技术,可简便、快速、准确、原位、直观地确定标记花生根瘤菌株形成的根瘤,从而方便地研究标记菌株与土著根瘤菌的竞争结瘤能力。无氮水培试验表明,标记菌株gusA4-5、gusA2-9分别与土著菌混和接种占瘤率为71.4%、77.0%。盆栽试验表明,接种供试菌株Spr4-5、Spr2-9占瘤率分别为57.9%、63.0%,比对照极显著增产52.5%、22.7%;接种Spr4-5比Spr2-9极显著增产24.2%。初步说明两个供试菌株的竞争结瘤力比土著根瘤菌强,菌株Spr2-9强于Spr4-5;Spr4-5比Spr2-9有效性高,是结瘤适量,竞争结瘤能力强的高效菌株。
GUS gene was transferred into the tested Bradyrhizobium sp. （Arachis） strains Spr4- 5 and Spr2 - 9 using GUS gene making technique. Nodules which occupied by the gus - marked strains could be evaluated in situ.. It was convenient for studying competitive nodulation between the gus - marked strain and the soil indigenous rhizobia. The N - free solution pot indicated that two tested trains had higher nitrogen f^ng efficiency and stronger competitive ability than of the soil indigenous bradyrhizobia. The rate of nodule occupancy of gus A2 - 9, gus A4 - 5 on peanut mot was 77.0%, 71.4%, respectively. The pot experiment indicated the rate of nodules formed by Spr4 - 5, Spr2 - 9 were 57.9%, 63.0% , and the yield increased by 52.5%, 22.7% compared with CK, respectively. The yield of inoculating Spr4 - 5 increased by 24.2% compared with Spr2 - 9. The increased yield reached significant level. Spr4 - 5 was good super strain in nodulation and nitrogen fixing efficency.
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2006年第3期51-53,共3页
Soil and Fertilizer Sciences in China
基金
该研究由"973"(2001CB108905)及赫尔辛基大学合作项目资助
关键词
GUS基因标记
慢生花生根瘤菌
竞争结瘤
GUS gene marking
Bradyrhizobium sp. （Arachis）
competitive nodulation
