摘要
目的:探讨谷氨酸对体外原代培养的大鼠耳蜗螺旋神经节细胞(SGC)兴奋性毒性作用。方法:SGC在体外培养4d,加入1mmol/L谷氨酸,继续培养24h后,用荧光显微镜观察Annexin-V-FITC和PI双标的细胞形态改变,以及用激光共聚焦方法观察细胞内游离钙离子浓度的变化。结果:体外培养4d的SGC,用1mmol/L谷氨酸处理24h后,与对照组相比,出现大量的细胞受损,表现为死亡或凋亡(P<0.05);用Fluo-3染色,激光共聚焦显微镜观察,加入谷氨酸后,80%的SGC细胞内钙离子迅速增加,150s达到高峰。结论:谷氨酸诱导的SGC钙离子内流与细胞受损有明显的关系。
Objective：To explore the excitotoxic effects of glutamate on the in vitro primarily cultured spiral ganglion cell （SGC） of rat. Method：Spiral ganglion cells （SGCs） were cultured in vitro for 4 days, and exposed to lmmol/L glutamate for 24 hours. Damaged cells double-labeling with Hoechest33258 and PI were observed by fluorescence microscope. Ffluo-3 and CLSM for measurement of intracellular calcium levels were also utilized. Result： Most cells were damaged and the intracellular calcium increased and after exposure to 1 mmol/L glutamate（ P〈 0.05）. ConclusiontGlutamate excitotoxicity was associated with free intracellular calcium ion concentration elevation in SGC.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第13期611-613,共3页
Journal of Clinical Otorhinolaryngology
基金
国家自然科学基金资助项目(No:30471879)
