摘要
从石蜡包埋胃癌组织标本中提取ＤＮＡ，用ＰＣＲ扩增Ｐ（５３）基因第７外显子，经ＨａｅⅡ酶切发现Ｐ（５３）基因突变，免疫组组化学观察、分析４２例胃癌生物学行为，结果Ｐ（５３）基因突变产物表达与患者年龄、性别、溃疡大小、肉眼类型、组织学类型、浸润深度之间均无相关关系（Ｐ＞０．０５），仅与淋巴结转移有显著相关（Ｐ＜０．０１），提示Ｐ（５３）基因突变与胃癌细胞异常增殖有关。
The DNA was extracted from the paraffin block of gastric carcinoma and the seventh exon of P(53) oncogene was amplificated by the polymerase chain reaction (PCR).The mutation of P(53)oncogene was found by digestion with Hae Ⅱ. The biological behavior in 42 cases of gastric carcinoma was examined by the avidin-biotin complex (ABC) method.The results showed that the mutation of P(53) oncogene was not related with the patient's age,sex,size of ulcer, gross and histologicaI type, and depth of infiltration (P>0.05),and onlycorrelated with the metastasis of lymph node. It is concluded that the mutation of P(53) oncogene is related with the unusual increasing of gastric carcinoma cells.
出处
《延边医学院学报》
CAS
1996年第3期128-131,共4页
Journal of Medical Science Yanbian University
