摘要
目的:骨形态发生蛋白12属于转化生长因子β超家族成员,能诱导间充质干细胞定向分化为成腱细胞。重组人骨形态发生蛋白12不但能使腱纤维原细胞的增殖,还能使Ⅰ型和Ⅲ型原胶原高表达,有助于肌腱的修复和愈合。回顾骨形态发生蛋白12的发展及其研究成果,并对其综述。资料来源:应用计算机检索NCBIEntrezPubmed和CNKI1965-01/2004-12骨形态发生蛋白12的相关文献,检索词:骨形态发生蛋白12(bonemorphogeneticprotein-12),损伤(repairing)。资料选择:对资料进行初审,纳入标准:①有关骨形态发生蛋白12生物学效应。②有关骨形态发生蛋白12与其家族成员比较研究。排除标准:重复研究。资料提炼:共收集42篇相关文献,排除重复或类似的同一研究,28篇符合研究要求。资料综合:①骨形态发生蛋白12的生物学作用:能诱导间充质干细胞定向分化为成腱细胞。重组人骨形态发生蛋白12不但能使腱纤维原细胞的增殖,还能使Ⅰ型和Ⅲ型原胶原高表达,有助于肌腱的修复和愈合。②与其家族其他成员比较:骨形态发生蛋白12和骨形态发生蛋白-2的信号转导途径不同;虽然骨形态发生蛋白13具有近似骨形态发生蛋白12的作用,能促进成腱细胞的增殖和Ⅰ型原胶原的基因表达,但对Ⅲ型原胶原的基因表达作用不显著。结论:成功克隆骨形态发生蛋白12基因,将其用于腱损伤的修复,具有重要临床应用价值,但目前对于骨形态发生蛋白12诱导骨髓间充质干细胞定向分化为腱细胞的机制尚未阐明,还有待进一步的研究。
OBJECTIVE： Bone morphogenetic protein-12, as one member of the transforming growth factor-β superfamily, can induce the mezenchymal stem ceils differentiating into bending ceils. Recombinant human bone morphogenetic protein-12 can increase proliferation of tendon fibroblasts and the gane expressions of procollagen type Ⅰ and type Ⅲ, which is helpful to the repairing and healing of tendon tissues. In this paper, we review and summarize the development and achievement of the bone morphogenetic protein-12. DATA SOURCES： The computer-basnd online search of NCBI Entrez Pubmed and CNKI were undertaken for literatures about the bone morphogenetic protein-12 published between January 1965 and December 2004 with the key word of ＂bone morphogenetic protein-12, repairing＂. STUDY SELECTION： The data were checked up primarily. Inclusive criteria： （1）Articles about biological effect of bone morphogenetic protein- 12; （2）Stndies on difference between bone morphoganetic protein-12 and other member in the family. Exclusive criteria： repetitive study. DATA EXTRACTION： A total of 42 relevant literatures were collected, those repetitive study or similar studies were excluded, and 28 articles met the inclusive criteria. DATA SYNTHESIS： （1）Biological effect of bone morphogenetic protein- 12： It can induce mezenchymal stem ceils differentiating into bending cells. Recombinant human bone morphogenetic protein-12 can increase proliferation of tendon fibroblasts and the gene expressions of procollagen type Ⅰ and type Ⅲ, which is helpful to the repairing and healing of tendon tissues. （2）Comparison between bone morphogenetic protein-12 and other member in the family： There are different conductive ways between bone morphogenetic protein-12 and bone morphogenetic protein-2; although bone morphogenetic protein-13 has the same biological effect to bone morphogenetic protein-12, which can increase proliferation of tendon fibroblasts and the gene expression of procollagen type Ⅰ, it has not obvious effect on the expression of procollagen type Ⅲ. CONCLUSION： As the successful clone of bone morphogenetic protein- 12, it plays an important role in repairing tendon tissues and clinical application. But the mechanism of differentiation of bone morphogenetic protein-12 into bending cells has not been clarified; therefore, it need further study.
出处
《中国临床康复》
CSCD
北大核心
2006年第25期150-152,共3页
Chinese Journal of Clinical Rehabilitation
