摘要
目的:观察100dB条件下不同频率次声波对小鼠成骨样细胞增殖和分泌功能等生物学特性的影响。方法:实验于2003-03/06在解放军第四军医大学放射生物学教研室完成。复苏培养后的细胞随机分为空白对照组、4Hz100dB组、12Hz100dB组、20Hz100dB组。将冻存的细胞株复苏,选择对数生长期的细胞,以1×107L-1细胞浓度接种在48孔培养板中,共接种4板并确定细胞贴壁。次日起每天上午8时将各组细胞放入次声仓内,4Hz100dB组、12Hz100dB组、20Hz100dB组分别给予相应频率和声压级参数的次声作用,空白对照组在次声仓内无次声输出,30min/d,共5d。5d后进行细胞增殖计数,观察次声波作用后小鼠成骨样细胞碱性磷酸酶活性和骨钙素表达的变化。结果:①不同参数的次声波对小鼠成骨样细胞增殖的影响:小鼠成骨样细胞的形态为不规则的多角形,受次声波作用后形态无明显改变。次声波作用第5天,4Hz100dB组、12Hz100dB组,20Hz100dB组小鼠成骨样细胞密度均明显高于空白对照组犤(52.25±8.52),(50.33±7.99),(49.82±7.05),(39.92±7.38)107L-1,P均<0.05犦。②次声波作用后小鼠成骨样细胞骨钙素的表达情况:次声波作用5d后,4Hz100dB组、12Hz100dB组、20Hz100dB组小鼠成骨样细胞骨钙素浓度均明显高于空白对照组犤(1.4012±0.5319),(0.8775±0.2589),(2.2575±0.8307),(0.5925±0.1725)μg/L;P=0.001,0.023,0.0005犦。③次声波作用后小鼠成骨样细胞碱性磷酸酶活性的变化:次声波作用5d后,各组的小鼠成骨样细胞碱性磷酸酶表达均较低,4Hz100dB组、12Hz100dB组、20Hz100dB组与空白对照组的吸光度值基本相似(0.016±0.008,0.013±0.005,0.014±0.005,0.013±0.005,P=0.92,1.0,0.95)。结论:4,12Hz100dB次声波作用可明显促进细胞增殖,20Hz100dB次声波作用具有显著分泌骨钙素的作用。提示100dB次声波不同频率下可以促进成骨样细胞的体外增殖和分泌功能。
AIM： To observe the effects of infrasound of different frequencies under 100 dB on biological characteristics such as proliferation and secretaries function etc. of oeteoblast-like cells in mice. o METHODS： The experiment was conducted in the Department of Radiobiology of Fourth Military Medical University of PLA from March to June 2005. After primary culture, cells were randomly divided into blank control group, 4 Hz 100 dB group, 12 Hz 100 dB group and 20 Hz 100 dB group. Frozed cell stains reanimated and cells in logarithmic growth phase were selected and inoculated into 48-pore cultivating board at the concentration of 1×10^7L^-1. A total of 4 boards were inoculated into and cells were adhere to the walls. From 8 PM of the next day, cells of each group were exposed to infrasound. Cells in the 4 Hz 100 dB group, 12 Hz 100 dB group and 20 Hz 100 dB group were exposed to infrasound at corresponding frequencies and that of sound pressure level （SPL） parameter;, Cells in the blank control group were exposed to infrasound without output for 5 days and 30 minutes each day. Cellular proliferation was calculated, and alkaline phosphatease activities （APA） and changes of expression of oeteocalcin （OC） in osteoblast-like cells of mice exposed to infrasound were observed after 5 days. RESULTS： （1）Effects of infrasound of different parameters on oeteoblastlike cells of mice： Osteoblast-like cells were in irregular polygons, which did not changed obviously after being exposed to infrasound. 5 days after exposure, densities of oeteoblast-like cells in t/flee of 4 Hz 100 dB group, 12 Hz 100 dB group and 20 Hz 100 dB group were remarkably higher than those in the blank control group [（52.25±8.52）, （50.33±7.99）, （49.82±7.05）, （39.92±7.38）10^7 L^-1,P 〈 0.05]. （2）Expressions of OC in osteoblast-like cells of mice after being exposed to infrasound： At 5 days after exposure, concentrations of OC of osteoblast-like cells in mice of 4 Hz 100 dB group, 12 Hz 100 dB group and 20 Hz 100 dB group were markedly higher than those in the blank control group [（1.401 2±0.531 9）, （0.877 5 ±0.258 9）, （2.257 5±0.830 7）, （0.592 5±0.172 5） p.g/L;P=0.001, 0.023,0.000 5]. （3）Changes of APA in osteoblaat-like cells of mice after being exposed to infrasound： 5 days after exposure, expressions of APA in oeteoblast-like cells of each group were lower, and absorption value in 4 Hz 100 dB group, 12 Hz 100 dB group, 20 Hz 100 dB group and blank control group were essentially similar （0.016 ±0.008,0.013±0.005,0.014±0.005, 0.013±0.005, P=0.92,1.0,0.95）. CONCLUSION： Infrasound of 4,12 Hz 100 dB can significantly promote cell proliferation, and that of growth and 20 Hz 100 dB has an marked effect on secretion of OC, which suggests that in different frequency,proliferation in vitro and secretion function of osterblast-like cells can be promoted by being exposed to infrasound of 100 dB.
出处
《中国临床康复》
CSCD
北大核心
2006年第25期83-85,共3页
Chinese Journal of Clinical Rehabilitation
