摘要
目的:观察不同强度恒磁场辐射对人脐血间充质干细胞增殖水平及细胞周期的影响。方法:实验于2005-08/2005-10在中南大学湘雅医学院组织胚胎学教研室完成。①用密度梯度离心法分离人脐血间充质干细胞。②经贴壁筛选法筛选,对生长良好的第3代人脐血间充质干细胞进行恒磁场辐射(强度分别为0.05,0.1,0.5和1.0mT),连续刺激5d,8h/d。③用四甲基偶氮唑盐法、流式细胞仪法测定细胞增殖、细胞周期和细胞凋亡情况。结果:①0.05mT组细胞增殖显著高于对照组(0.198±0.011,0.162±0.007,P<0.05);细胞周期静止期/DNA合成前期比例显著降低(66.3±0.1,75.8±0.3,P<0.05);DNA合成期和DNA合成后期/分裂期比例显著增加(14.9±0.3比9.1±0.1,18.8±0.2比15.1±0.1,P<0.05)。②0.1mT组细胞增殖和细胞周期与对照组相比无明显差异。③0.5mT组和1.0mT组细胞增殖均显著低于对照组,细胞周期静止期/DNA合成前期比例显著增加,DNA合成期和DNA合成后期/分裂期比例显著降低(P<0.05)。④各组均未发现DNA倍体异常细胞。结论:恒磁场对人脐血间充质干细胞增殖的影响与磁感应强度有关,0.05mT的磁场促进间充质干细胞的增殖,0.1mT的磁场对间充质干细胞无明显影响,0.5mT和1.0mT的磁场抑制间充质干细胞的增殖。
AIM： To observe the effect of constant magnetic fields on the proliferation of human umbilical cord blood mesenchymal stem cells （UCBMSCs） and cellular cycle. METHODS： This experiment was conducted at the Department of Histology and Embryology, Xiangya Medical College, Central South University from August 2005 to October 2005. （1）UCBMSCs were separated with density gradient centrifugation. （2） The well-grown UCBMSCs of the third generation were irradiated by CMF following adherent screening method at different intensities （ The intensity was 0.05,0.1,0.5 and 1.0 mT respectively） for 5 days , 8 h/d. （3）Cellular proliferation, cycle and apoptosis were measured with methylthiazolyl tertrazolium （MTT） assay and flow cytometer. RESULTS： （1）Cellular proliferation was significantly higher in 0.05mT group than in the control group （0.198±0.011 vs 0.162±0.007,P 〈 0.05）; The proportion of G0/G1j of the cellular cycle decreased significantly （66.3±0.1 vs 75.8±0.3,P 〈 0.05）;tbe proportion of S phase and G2/M phase of the cell cycle increased significantly （ 14.9±0.3 vs 9.1±0.1,18.8 ±0.2 vs 15.1±0.1 ,P 〈 0.05）. （2） There were no significant differece in cellular proliferation and cell cycle between 0.10 mT group and control group. （3） Cellular proliferation was significantly lower in 0.50 mT group and 1.00 mT group than in the control group. The proportion of G0/G1 of the cell cycle increased significantly, the proportion of S phase and G2/M phase of the cell cycle decreased significantly when CMF intensity was 0.50 mT and 1.00 mT. （4）No abnormal ploidy was found in any group. CONCLUSION： The effect of CMF on the proliferation of UCBMSCs is dependent on the magnetic intensity. 0.05mT CMF can accelerate the proliferation of MSCs. 0.10 mT CMF has no effects on the growth of MSCS. However, 0.50 mT and 1.00 mT CMF can attenuate the growth of MSCS.
出处
《中国临床康复》
CSCD
北大核心
2006年第25期14-16,共3页
Chinese Journal of Clinical Rehabilitation
基金
湖南省卫生厅科研基金项目(B2005-117)~~
