摘要
目的:建立咪喹莫特固体脂质纳米粒包封率的测定方法。方法:采用热乳匀法制备咪喹莫特固体脂质纳米粒。用葡聚糖凝胶柱色谱法分离含药固体脂质纳米粒与游离药物,以蒸馏水和1．0×10-3mol·L-1盐酸溶液为洗脱液,用HPLC法测定游离药物量。结果:凝胶柱色谱法能够将包封药物和游离药物分开。游离咪喹莫特在0．335-2．69μg·ml-1浓度范围内,线性关系良好(r=0．999 9)。游离药物柱回收率为98．6％,柱的加样回收率为97．7％。样品的平均包封率为(51．43±0．88)％。结论:该方法操作简便,结果准确,可用于咪喹莫特固体脂质纳米粒包封率测定。
Objective： To determine the entrapment efficiency of solid lipid nanoparticles loading imiquimod. Method： The hot homogenization was used to prepare solid lipid nanopartieles of imiquimod. The solid lipid nanoparticles of imiquimod and free drug were separated by Sephadex G-50 chromatography. The mobile phase was distilled water and 1.0×10^3mol·L^-1 hydrochloric acid solution. The wavelength of detecting free imiquimod was at 244 nm. Result： Linear in the range was 0. 335-2.69μg·ml^-1 （r =0.999 9）. The recovery of column or somple was 98.6% and 97.7%, respectively. The entrapment efficiency of solid lipid nanoparticles of imiquimod was （51.43 ± 0.88 ） %. Conclusion： The method for determining is convenient, sensitive, accurate and suitable for determining the entrapment efficiency of the solid lipid nanoparticles loading imiquimod.
出处
《中国药师》
CAS
2006年第7期599-602,共4页
China Pharmacist
关键词
咪喹莫特
固体脂质纳米粒
包封率
Imiquimod
Solid lipid nanoparticles
Entrapment efficiency
