摘要
目的 检测人脑胶质细胞瘤中脆性组氨酸三联体基因（FHIT）、第10号染色体同源丢失性磷酸酶-张力蛋白基因（PTEN）的表达情况.方法 采用S-P免疫组织化学法.结果 FHIT和PTEN阳性染色定位于细胞浆,52例胶质瘤标本中,有39例FHIT呈阳性表达,低度恶性肿瘤（Ⅰ～Ⅱ级）的阳性表达率明显高于高度恶性（Ⅲ～Ⅳ级）肿瘤（P 〈0.05）;而有37例PTEN呈阳性表达,低度恶性肿瘤的阳性表达率明显高于高度恶性肿瘤（P〈0.05）.FHIT与PTEN在脑胶质瘤细胞中的表达呈正相关.结论 FHIT与PTEN的表达与脑胶质瘤的发生、发展密切相关,联合检测FHIT、PTEN在胶质瘤中的表达有助于对肿瘤细胞增殖能力、分化程度做出正确评价.
Objective To investigate the expression of FHIT and PTEN in human glioma. Methods We detect the expression of FHIT and PTEN by immunohistochemieal means. Results The FHIT and PTEN positive stainings were found in the eytoplasm. The total positive rate of FHIT was 75. 0% （39/52）. Positive rate has signifieant differenee in tumor pathological grades（ Ⅰ°～Ⅱ°and Ⅲ°～Ⅳ°） （P〈0. 05）. The positive rate decreased with progression of pathological grade. The total positive rate of PTEN was 71.2% （37/52）. Positive rate has also signifieant differenee in tumor pathological grades （P〈0. 05）. An obverse correlation was found between FHIT and PTEN expression in glioma. Conclusion The expression of FHIT and PTEN is elosely connected with the oceurrenee and development of glioma. The detection of expression of FHIT and PTEN in glioma may be helpful to evaluate the state of eell proliferation and the degree of proliferation.
出处
《华中医学杂志》
2006年第3期163-164,202,共3页
Central China Medical Journal
