摘要
目的探讨2型糖尿病(T2DM)抗血管紧张素Ⅱ的1型受体(AT1)和肾上腺素能α1(Adrα1)受体自身抗体与肾功能不全的关系。方法以合成的AT1和Adrα1受体多肽片段为抗原,应用ELISA技术,检测糖尿病肾病(DN)79例,T2DM52例,40例正常人血清中抗AT1和Adrα1受体自身抗体。结果DN组AT1和Adrα1受体自身抗体阳性率分别为45.6%和32.9%,明显高于T2DM组的13.5%和9.6%及正常对照组的10.0%、12.5%,差异有统计学意义(P<0.01)。结论抗G蛋白偶联AT1和Adrα1受体自身抗体可能与DN的发病有关。
Objective To explore the role of the autoantibodies against angiotensin Ⅱ type 1 receptor （AT1-receptor） and α1-adrenergic receptor（el-receptor）, in the development of diabetic nephropathy. Methods The epitopes of the second extracellular loop of AT1 receptor （165-191）, α1 receptor （192-218） were synthesized and used respectively to screen sera autoantibodies from patients with diabetic nephropathy （DN,n= 79）, diabetes mellitus without renal failure （DM, n= 52） and healthy blood donors （n=40, control） by ELISA. Results In DN patients, the positive rates of the autoantibodies against ATe-receptor and α1-receptor were 45.6% and 32.9%, respectively, which were all higher than those in patients with DM without renal failure （13.5% and 9.6% respectively） （P〈0. 01）, and in healthy donors （10% and 12.5% respectively） （P〈0.01）. Conclusion The autoantibodies against ATe-receptor and α1-receptor may play important roles in the pathogenesis of the diabetic nephropathy.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2006年第3期185-187,共3页
Chinese Journal of Diabetes
关键词
糖尿肾病
受体
肾上腺素能α1
受体
血管紧张素
自身抗体
Diabetic nephropathy
Receptors, adrenergic alpha-1
Receptors, angiotensin
Autoantibodies
