摘要
利用生物信息学方法结合实验手段克隆了一个在睾丸组织中特异高表达的小鼠生精细胞凋亡相关基因Spata17的人类同源基因SPATA17(GenBank登录号为AY963797)。应用生物信息学分析显示该基因定位在染色体1 q41,包含11个外显子,内含子和外显子交界区均符合gt-ag规则;该基因开放阅读框为1083 bp编码一个由361个氨基酸组成的、分子量为43.49 kD、等电点为9.71、含有三个保守IQ功能域的蛋白;对SPATA17编码蛋白质进行生物信息学分析,无跨膜区,无信号肽序列,推测为一非分泌性蛋白。多组织和Northern b lot结果显示该基因只在睾丸组织中特异高表达,转录本大小为2.0 kb。总之,研究表明SPATA17在睾丸组织生精细胞凋亡起重要作用。
SPATA17 gene, homologne to mouse MSRG-11 gene, was cloned by informatics analysis. The cDNA mapped to chromosome 1 q41 in the human genome is made up of 11 exons and the exon-intron boundaries obey to the AG/GT rule. The gene contains a 1083 bp open reading frame encoding 361 amino acid sequences with theoretical molecular weight of 47.49 KD and isoelectric point of 9.75. The protein encoded by this gene contains three highly conserved IQ motifs and is a new member of IQ protein family. According to the computer-aided analysis of the putative protein encoded by the SPATA17, no transmembrane region and no signal peptides are found in the protein. Muhi-tissue RT-PCR and northern results show that the SPATA17 transcript of 2.0 kb was detected strongly in adult human testis only. In conclusion, this study predicts its important role in spermatogenesis cell apoptosis
出处
《激光生物学报》
CAS
CSCD
2006年第3期283-287,共5页
Acta Laser Biology Sinica
基金
TheMajorStateBasicResearchDevelopmentProgramofChina(G1999055901)
