摘要
为探讨谷胱甘肽－Ｓ－转移酶Ｍ１基因５＇端非编码区ＣＧＧ三核苷酸重复序列长度多态与肝癌遗传易感性的关系，采用ＰＣＲ－ＰＡＵＧＥ－ＤＮＡ银染方法，对６５例肝癌患者和１０６例健康对照的研究表明，病例组和对照组ＣＧＧ拷贝数小于６者，分别占４９．２３％和７．５５％，两组差异显著（Ｐ＜０．０００１）。ＯＲ值为１１．８８，ＥＦ值为０．４５０９．提示ＣＧＧ拷贝数小于６者，患肝癌的危险性增加１０．８８倍，由ＣＧＧ拷贝数小于６所致的肝癌病例占人群中全部肝癌病例的４５．０９％。
In order to research the relationship between the length polymorphism of CGG trinucleotide repeat sequence located in the 5'-untranslated region(5'-UTR) of GSTM1 gene and the genetic susceptibility to primary hepatocellular carcinoma(PHC),65 cases with PHC and 106 healthy controls were studied by using PCR-PAUGE-DNA silver staining techniques. The results show that the percentages of those having the CGG repeats less than 6 copies among the cases and the controls are 49.23% and 7.55% respectively. The difference is statistically significant(P<0.0001), The odds ratio(OR) is 11.88, and the etiological fraction(EF)is 0. 4509. The results suggest that the CGG repeats less than 6 copies contribute a 10. 88 times of the increased risk of PHC, and 45. 09% of the PHC cases in the population can be ascribed to the CGG repeats less than 6 copies.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1996年第3期135-137,共3页
Chinese Journal of Medical Genetics
基金
国家自然科学基金
关键词
肝肿瘤
GSTM1
基因多态性
三核苷酸
遗传易感性
Primary hepatocellular carcinoma
GSTM_1 gene
Trinucleotide repeat sequence
Genetic suscetibility
